Rna Detection
Rna Detection - Related Videos
02:42
RNA CISH Signal Detection: A Technique to Detect Chromogenic Signals During RNA In Situ Hybridization in Intact Tissue Specimens
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2023
This video presents the protocol for signal detection during RNA chromogenic in situ hybridization. This technique can be used to diagnose an active oncogenic infection visually in an intact tissue specimen.
10:34
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
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Cited by 19 •
2016
An RNA pull-down protocol is optimized here for detection of interactions between RNA-binding proteins (RBPs) and noncoding as well as coding RNAs. An RNA fragment from androgen receptor (AR) was used as an example to demonstrate how to retrieve its RBP from lystate of primary brown adipocytes.
08:50
A Nonsequencing Approach for the Rapid Detection of RNA Editing
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2022
Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.
10:16
Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Cited by 35 •
2012
A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.
06:36
Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
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Cited by 6 •
2017
Native polyacrylamide gel electrophoresis is a fundamental tool for analyzing RNA-protein interactions. Traditionally most experiments have used vertical gels. However, horizontal gels provide several advantages, such as the opportunity to monitor complexes during electrophoresis. We provide a detailed protocol for generating and using horizontal native gel electrophoresis.
FAQs
Sequence specificity comes from complementary base pairing between a target RNA sequence and a matching probe or primer. Only molecules with sufficiently corresponding sequences are expected to bind or support downstream analysis. This principle allows researchers to distinguish particular transcripts within complex biological samples and connect measured signals to defined genes or RNA targets.
Reverse transcription converts RNA into complementary DNA, creating a DNA form that can be amplified and analyzed. This step is central to reverse transcription PCR because the resulting complementary DNA provides the template for primer-directed amplification. The conversion therefore links RNA molecules to analytical methods designed to measure amplified nucleic-acid products.
These methods emphasize different types of information. Reverse transcription PCR supports analysis of transcript abundance after conversion to complementary DNA and amplification. Fluorescence in situ hybridization shows where particular RNA molecules occur in cells or tissues. RNA sequencing additionally provides sequence information, making the approaches complementary rather than interchangeable.
Depending on the technique, RNA detection can indicate how much of a transcript is present, where it is located, or what sequence it contains. These distinctions help connect RNA measurements with cellular states and gene activity. They also allow investigators to examine disease-associated changes or responses to environmental and therapeutic conditions.
A typical workflow begins with a biological sample containing RNA, followed by reverse transcription to generate complementary DNA. Sequence-specific primers then guide amplification of the target-derived material, and the amplified product is analyzed to assess the transcript. This sequence of steps converts an RNA measurement into an amplification-based result.
Fluorescence in situ hybridization is especially useful when the location of RNA within cells or tissues matters. Fluorescent, sequence-specific probes bind matching RNA molecules, allowing their spatial distribution to be examined. This capability adds cellular context that an abundance measurement alone may not provide, which is valuable in developmental and disease-related biology.
Biologists apply RNA detection to investigate gene regulation, development, infection, cancer, and responses to environmental or therapeutic conditions. Measurements can reveal shifts in transcript abundance, cellular localization, or sequence across experimental states. Comparing these RNA-associated changes helps researchers relate molecular activity to broader biological processes and disease-associated cellular behavior.