Srebp-1 Activation
Srebp-1 Activation - Related Videos
10:34
Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
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Cited by 11 •
2017
This report describes an in vitro assay for measuring the human immunodeficiency virus type 1 (HIV-1) preintegration complex (PIC)-associated integration activity in the cytoplasmic extracts of acutely infected cells. The integration of PIC-associated HIV-1 DNA into heterologous target DNA is quantified by using a nested real-time polymerase chain reaction strategy.
07:20
Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)
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Cited by 9 •
2021
Proteins and amine-containing ligands can be covalently linked to polysaccharides activated by the cyanylation reagent, 1-cyano-4-dimethylaminopyridine tetrafluoroborate (CDAP), to form covalent protein (ligand)-polysaccharide conjugates. This article describes an improved protocol for carrying out controlled CDAP activation at 0 °C and varying pH and performing subsequent conjugation of the activated polysaccharides.
08:11
Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
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Cited by 1 •
2021
These protocols provide the methodology used to assess the enzymatic activity of individual members of the protein arginine methyltransferase (PRMT) family in cells. Detailed guidelines on assessing PRMT activity using endogenous and exogenous biomarkers, methyl-arginine recognizing antibodies, and inhibitor tool compounds are described.
11:22
Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1
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Cited by 3 •
2017
In this two-part study, a biological actuator was developed using highly flexible polydimethylsiloxane (PDMS) cantilevers and living muscle cells (cardiomyocytes), and characterized. The biological actuator was incorporated with a base made of modified PDMS materials to build a self-stabilizing, swimming biorobot.
10:07
Growing Magnetotactic Bacteria of the Genus Magnetospirillum: Strains MSR-1, AMB-1 and MS-1
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Cited by 17 •
2018
We present a procedure for growing several strains of Magnetospirillum in two different types of growth media. Magnetospirillum gryphiswaldense strain MSR-1 is grown in both liquid and O2 concentration gradient semi-solid media while M. magneticum strain AMB-1 and M. magnetotacticum strain MS-1 are grown in liquid medium.
FAQs
Both signals can promote the pathway, but they represent different metabolic conditions. Insulin indicates a regulatory state associated with increased lipogenic activity, whereas reduced sterol availability reflects a shortage of sterol-related lipid components. In either case, the relevant outcome is movement of the SREBP-1 precursor from the endoplasmic reticulum toward the Golgi for processing.
The two proteases provide sequential processing steps that convert the membrane-associated precursor into a form capable of regulating transcription. Site-1 and site-2 protease activity occurs in the Golgi after precursor transport from the endoplasmic reticulum. Without this cleavage sequence, the transcriptionally active N-terminal portion would not be released to influence lipogenic gene expression.
Nuclear entry changes SREBP-1 from a processed precursor into a direct transcriptional regulator. The released N-terminal factor binds sterol regulatory elements in target genes, connecting the earlier membrane-trafficking and cleavage events to gene expression. This step is important because it translates metabolic signals into increased transcription of genes involved in fatty acid and triglyceride synthesis.
The precursor is associated with the endoplasmic reticulum before transport and cleavage, while the processed N-terminal fragment can enter the nucleus. This difference separates signal handling from transcriptional control within the pathway. Studying both forms helps distinguish whether a change affects precursor movement, proteolytic processing, nuclear localization, or the final regulation of lipogenic genes.
A pathway-focused study can follow the process in order: identify the metabolic condition, examine precursor movement from the endoplasmic reticulum to the Golgi, assess site-1 and site-2 protease processing, and then evaluate nuclear entry and sterol regulatory element binding. Linking these stages helps determine where regulation occurs and how it affects lipogenic gene transcription.
The pathway provides a mechanistic link between metabolic signals and the expression of genes that produce fatty acids and triglycerides. Because those lipid-synthesis programs are relevant to lipid homeostasis, altered regulation can be examined in the context of obesity, fatty liver disease, and diabetes. Research can therefore use SREBP-1 activation to connect cellular signaling with metabolic disease biology.