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JoVE Journal
Immunology and Infection

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Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
 

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes

Article doi: 10.3791/54581
February 22nd, 2017 Usage Statistics

Summary February 22nd, 2017

This report describes an in vitro assay for measuring the human immunodeficiency virus type 1 (HIV-1) preintegration complex (PIC)-associated integration activity in the cytoplasmic extracts of acutely infected cells. The integration of PIC-associated HIV-1 DNA into heterologous target DNA is quantified by using a nested real-time polymerase chain reaction strategy.

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