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JoVE Journal
Bioengineering
Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot – PART 1
Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot – PART 1
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot – PART 1

Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot – PART 1

Full Text
8,487 Views
11:22 min
July 11, 2017

DOI: 10.3791/55642-v

Merrel T. Holley*1, Neerajha Nagarajan*2, Christian Danielson1, Pinar Zorlutuna*2, Kidong Park*1

1Division of Electrical and Computer Engineering,Louisiana State University, 2Department of Aerospace and Mechanical Engineering, Bioengineering Graduate Program,University of Notre Dame

In this two-part study, a biological actuator was developed using highly flexible polydimethylsiloxane (PDMS) cantilevers and living muscle cells (cardiomyocytes), and characterized. The biological actuator was incorporated with a base made of modified PDMS materials to build a self-stabilizing, swimming biorobot.

The overall goal of this video is to describe how to develop and characterize a biological actuator using flexible PDMS cantilevers in cardiomyocytes. These actuators are then incorporated with a modified base to form a self-stabilizing swimming biorobot. The verification method described here is unique, wherein the mechanical base structure is modified to form a self-stabilizing swimming biorobot whose position can be also determined externally with magnets.

The main advantage of this technique is that the base properties can be easily altered to tune the buoyancy of the device and for self-stabilization. The method can be applied to develop other novel devices which would require self-stabilizations and flotation. Generally, individuals new to this method will struggle because mechanically deforming the cantilevers is challenging.

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