Dye Retention
Dye Retention - Related Videos
02:42
Determination of the Calcium Retention Capacity of Isolated Mitochondria
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2025
This video demonstrates the procedure to assess mitochondrial calcium retention capacity by monitoring real-time changes in fluorescence as mitochondria uptake calcium. This assay is useful for studying mitochondrial function and calcium-related processes, including apoptosis and neurodegenerative diseases.
05:53
Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
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Cited by 11 •
2018
This protocol aims to describe a method to examine the Ca2+ retention capacity and Ca2+- triggered mitochondrial swelling of isolated mitochondria of SH-SY5Y cells step-by-step.
11:01
Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention
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Cited by 12 •
2015
Our goals were to design, manufacture and test ferromagnetic stents for endothelial cell capture. Ten stents were tested for fracture and 10 more stents were tested for retained magnetism. Finally, 10 stents were tested in-vitro and 8 more stents were implanted in 4 pigs to show cell capture and retention.
03:10
Soil Core Sample Collection for Water Retention Property Analysis
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2026
Source:Alessia J. Marchesan1, Guillermo Hernandez Ramirez11Renewable Resources, University of Alberta. This video demonstrates the technique for collecting a soil sample for hydraulic property analysis. The sampling area is cleared, and the top crust is evacuated. The sampling ring is then placed into the soil to collect the sample. The ring with the sample is then cleaned, labeled, packed, and transferred undisturbed for analysis.
08:36
FM Dyes in Vesicle Recycling
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2023
FM dyes are a class of fluorescent molecules that has found important use in studying the vesicle recycling process. By virtue of a chemical structure, these molecules can insert themselves into the outer leaflet of phospholipid bilayer membranes. After membrane insertion, they are internalized into the cell via endocytosed vesicles, and released when these vesicles recycle back to the membrane. Since, these dyes fluoresce strongly in the hydrophobic environment within membranes and weakly in...
FAQs
The measured signal reflects several linked processes rather than uptake alone. Cells may accumulate the dye, bind or trap it internally, transport it elsewhere, metabolize it, or release it. Because these processes can change during incubation, retention measurements are most informative when uptake and the subsequent time period are controlled consistently across cells, tissues, or experimental conditions.
Membrane integrity helps determine whether a dye remains inside a cell or escapes into its surroundings. Damage can increase leakage and reduce the retained signal, whereas intact membranes may support continued intracellular accumulation. Consequently, a lower measurement can reflect altered membrane condition as well as other changes in transport, metabolism, or intracellular trapping.
Treatment conditions may alter uptake, intracellular retention, membrane integrity, transport, or metabolism, producing measurable differences in signal. Comparing those measurements across treated and untreated cancer models can help assess drug responses and related changes in cell viability or damage. Consistent incubation and measurement conditions are important so observed differences reflect treatment effects rather than assay variation.
A typical workflow establishes dye uptake during a defined incubation, removes unbound or external dye through washing, and measures the signal that remains after a specified period. Researchers can then compare retained signal among samples or conditions. Recording the incubation and measurement timing is essential because retention changes as intracellular trapping, transport, metabolism, and leakage proceed.
Imaging-based measurements preserve spatial information that a single overall signal may obscure. They can reveal regions with different levels of retained dye within a heterogeneous tumor, helping researchers examine local variation across tumor tissue. This approach is particularly useful when labeled cells or tissues are distributed unevenly and location is relevant to interpretation.
These measurements can contribute to studies of cell viability, proliferation, membrane damage, and responses to drugs. They can also help examine the distribution of labeled tumor cells or tissues. Applying the same retention assay across cancer models and treatment conditions supports structured comparisons, while imaging can add information about spatial differences within those samples.