Mouse Tumor Samples
Mouse Tumor Samples - Related Videos
02:43
Tumor Implantation and Injection of a Tumor Vaccine in a Mouse Model
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2025
This video demonstrates a cell-based tumor vaccination assay. A mouse, intradermally injected with B16-F10 melanoma cells for tumor formation, is vaccinated with B16-F10 cells secreting Flt3L cytokine near the developed tumor. The secreted Flt3L from the vaccine promotes the maturation and proliferation of dendritic cells that then present tumor antigens to T cells in lymph nodes, triggering a targeted anti-tumor immune response.
01:29
Anti-Tumor Effects of Engineered Pediococcus pentosaceus in a Mouse Tumor Model
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2026
Begin with immunodeficient mice bearing subcutaneous xenograft tumors derived from human colorectal cancer cells.Orally administer Pediococcus pentosaceus, a lactic acid bacterium safe for consumption and engineered to secrete an anticancer protein, to the treated mouse.Administer saline to the control mouse.Being acid-tolerant, the bacteria survive the acidic environment of the stomach and reach the intestine.There, they colonize the intestinal lumen and secrete the anticancer protein.The...
03:59
Orthotopic Homograft Tumor Transplantation: A Technique to Generate Pancreatic Tumor Mouse Models
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2023
The video explains a stepwise surgical method for orthotopic implantation of a tumor fragment from a cancerous donor to the pancreas of a non-cancerous recipient mice for development of pancreatic cancer. Orthotopically implanted PDAC could recapture the tissue microenvironment of human pancreatic cancers than conventional subcutaneous (SC) models more accurately.
05:58
Dissociation of Human and Mouse Tumor Tissue Samples for Single-cell RNA Sequencing
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Cited by 4 •
2024
This protocol outlines the procedure for rapidly dissociating human and mouse tumor samples for single-cell RNA sequencing.
03:58
Brain Tumor Resection in a Mouse Using a Minimally Invasive Resection System
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2025
Source: Alomari, S., et al. Implementation of Minimally Invasive Brain Tumor Resection in Rodents for High Viability Tissue Collection. J. Vis. Exp. (2022)This video demonstrates the use of a minimally invasive resection system (MIRS) to excise tumor tissue from an anesthetized mouse implanted with a tumor. The procedure involves securing the mouse onto a stereotactic frame, reopening the previous surgical site to access the brain, and inserting a specialized cannula that utilizes suction and a...
FAQs
Preservation method determines which features remain available for analysis. Fixation supports examination of tissue structure under the microscope, whereas freezing helps retain material for molecular investigations. Processing into cell suspensions makes cellular-level studies possible. Selecting among these approaches therefore connects sample handling with the type of biological evidence researchers need from a tumor.
These analyses examine complementary aspects of tumor biology. Microscopy provides information about tissue structure, molecular studies assess gene activity, and cellular analysis evaluates properties of individual cells. Considering these results together can show how tumor features relate to one another and can clarify biological changes associated with tumor growth or experimental treatment.
Tumor behavior is studied in relation to the tissue surrounding it, rather than as an isolated cellular phenomenon. Mouse tumor samples can preserve or provide access to this broader context for structural and cellular examination. Including surrounding tissue helps researchers investigate interactions that may contribute to tumor biology and interpret findings within the tumor environment.
Comparing samples from different experimental groups helps distinguish treatment-related or condition-related changes from features shared across tumors. Researchers can examine differences in tumor growth, gene activity, structure, or cellular characteristics and relate those findings to the experimental design. This comparative approach strengthens interpretation of drug effects and treatment responses in cancer research.
A general workflow begins with collecting tissue or cells from mice bearing tumors, followed by preservation in a form suited to the planned investigation. Samples may be fixed, frozen, or processed into cell suspensions before microscopic, molecular, or cellular analysis. The selected preparation links the collected material to the measurements needed for the study.
They are useful when researchers need to connect tumor growth or treatment response with biological evidence inside the tumor. Analyses can support evaluation of biomarkers, gene activity, tissue structure, cellular features, and drug effects. Results from these samples help relate controlled laboratory observations to disease mechanisms and inform investigation of potential therapies.