Localized Gene Alteration
Localized Gene Alteration - Related Videos
16:19
Localized RNAi and Ectopic Gene Expression in the Medicinal Leech
0 Views •
Cited by 5 •
2008
In this video, we show a procedure for an accurate biolistic delivery of reagents into live tissue with a novel miniature gene gun. We are knocking down the expression of the axon guidance molecule Netrin in leech embryos by delivering molecules of dsRNA into the ventral body wall and ganglia of single segments.
11:52
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
0 Views •
Cited by 15 •
2016
We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.
06:21
Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
0 Views •
Cited by 12 •
2018
This protocol describes a newly established method for virus delivery to the murine prostate. Using either CRISPR/Cas9 technology, gene overexpression, or Cre recombinase delivery, the technique allows orthotopic alteration of gene expression and implements a novel mouse model for prostate cancer.
09:30
Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
0 Views •
Cited by 5 •
2017
This paper describes a detailed and highly effective RNA in situ hybridization protocol particularly for low-level expressed Odorant Receptor (OR) genes, as well as other genes, in insect antennae using digoxigenin (DIG)-labeled or biotin-labeled probes.
01:30
Alterations in Respiration II
0 Views •
2024
There are numerous types of normal and abnormal respiration. Based on ventilatory movements, breathing patterns are classified as regular, deep, or shallow. Examples include Biot's breathing, Cheyne-Stokes respiration, Kussmaul's breathing, hyperventilation, and hypoventilation. Each pattern is clinically significant and aids in evaluating patients. In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes include...
FAQs
The key mechanistic advantage of Localized Gene Alteration is the connection between one selected genomic site and a measurable cellular phenotype. An editing system recognizes the chosen locus, while the introduced change affects a coding region, regulatory element, oncogene, or tumor-suppressor gene. This linkage helps researchers investigate whether that alteration contributes to cancer-related cellular behavior.
The consequence depends on both the sequence change and its genomic context. Introducing, removing, or replacing nucleotides within a coding region may alter the information carried by that region, whereas modifying a regulatory element can affect gene control. Targeting an oncogene or tumor-suppressor gene focuses the experiment on genetic categories central to cancer research.
Restricting the change to a defined locus helps researchers relate a molecular alteration to specific cellular behaviors. The resulting effects may include changes in cell growth, survival, invasion, or response to treatment. This focused relationship supports functional studies by narrowing the connection between a candidate cancer-associated gene and an observed phenotype.
A study generally begins by selecting the genomic locus and deciding whether to introduce, remove, or replace nucleotides. Researchers then use a genome-editing system that recognizes that site and examine how the resulting alteration affects the cells or organism. The measured outcomes can include growth, survival, invasion, or treatment response.
Localized Gene Alteration supports cancer models that carry a selected change in a defined gene or regulatory region. Researchers can use these models to study disease-driving mutations and examine how they influence cellular behavior. Because the alteration is focused on a chosen locus, the model can connect a particular genetic event with cancer-relevant effects.
Researchers can use a defined genetic alteration to investigate whether a cancer-associated gene influences treatment response. By observing how cells with the selected change behave during treatment, studies can connect genotype with sensitivity or resistance-related outcomes. This application helps evaluate whether an oncogene, tumor-suppressor gene, or related locus represents a potential therapeutic target.