Lamina Propria Mononuclear Cells
Lamina Propria Mononuclear Cells - Related Videos
05:01
Lamina Propria Mononuclear Cell Isolation: A Silica-based Density Gradient Centrifugation Technique to Obtain Mononuclear Cells from Murine Colon
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2023
In this video, we demonstrate the isolation of lamina propria mononuclear cells from murine colonic tissue by enzymatic digestion using collagenase E and silica-based density separation media gradient separation. The isolated mononuclear cells can be used for subsequent downstream analysis.
09:48
Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
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Cited by 9 •
2019
The goal of this protocol is to isolate mononuclear cells that reside in the lamina propria of the colon by enzymatic digestion of the tissue using collagenase. This protocol allows for the efficient isolation of mononuclear cells resulting in a single cell suspension which in turn can be used for robust immunophenotyping.
04:52
Isolating Enteric Glial Cells From the Submucosa and Lamina Propria of a Mouse
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2025
This video demonstrates a method for isolating enteric glial cells from the submucosa and lamina propria of the mouse intestine. It outlines the steps involved in extracting submucosa and lamina propria tissue, isolating the enteric glial cells from them, and culturing the cells on a coated well-plate to establish an enteric glial cell culture.
07:53
Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
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Cited by 21 •
2018
Here, we describe the isolation of enteric-glial cells from the intestinal-submucosa using sequential EDTA incubations to chelate divalent cations and then incubation in non-enzymatic cell recovery solution. Plating the resultant cell suspension on poly-D-lysine and laminin results in a highly enriched culture of submucosal glial cells for functional analysis.
04:11
Mononuclear Cell Isolation from Mouse Brain: A Density Gradient Centrifugation Technique to Isolate Brain Resident Mononuclear Cells
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2023
In this video, we perform the isolation of mononuclear cells from a mouse’s brain using density gradient centrifugation. The isolated mononuclear cell suspension can be used for further downstream analysis.
FAQs
These cells connect local immune surveillance with inflammatory signaling in mucosal tissue. Their composition, including lymphocytes, macrophages, and dendritic cells, allows investigators to examine how different leukocyte populations contribute to tissue defense or inflammatory states. In cancer studies, comparing these responses with tumor-associated changes can clarify how inflammation interacts with disease progression.
Cellular composition determines which immune functions can be examined. Lymphocytes, macrophages, and dendritic cells provide distinct perspectives on antigen recognition, cytokine production, and communication with surrounding tissue. Identifying these populations helps researchers relate a measured immune response to particular leukocyte groups rather than treating all recovered cells as a single functional population.
Studying immune-cell and tumor-cell interactions provides information about how the local immune environment may influence cancer biology. The analysis can connect immune-cell infiltration with cytokine production, antigen recognition, and tumor-associated inflammation. In this way, lamina propria mononuclear cell studies help characterize the immune context surrounding mucosal tumors rather than examining tumor cells in isolation.
Cytokine production measurements describe inflammatory signaling, while antigen recognition analysis examines how immune cells respond to relevant targets. Together, these readouts help distinguish the presence of immune cells from their functional activity. In mucosal cancer research, they support investigation of whether local immune responses are associated with tumor-associated inflammation or with responses to therapeutic intervention.
The workflow begins by removing the epithelial layer, followed by enzymatic digestion of the underlying connective tissue. This releases leukocytes that can then be separated into a usable immune-cell population. The resulting preparation supports downstream examination of cellular composition, cytokine production, antigen recognition, and interactions with tumor cells in the relevant mucosal setting.
In colorectal cancer studies, these cells provide a way to investigate immune infiltration and tumor-associated inflammation within gastrointestinal tissue. Researchers can examine which leukocyte populations are present and assess their cytokine production, antigen recognition, or interactions with tumor cells. These measurements help connect local mucosal immune activity with mechanisms involved in colorectal cancer development.
Their value lies in capturing immune activity within the mucosal environment where gastrointestinal tumors develop. Researchers can evaluate immune-cell infiltration, cytokine production, antigen recognition, and communication with tumor cells to characterize treatment-associated responses. This context supports investigation of why an immunotherapy response may coincide with particular local immune features or inflammatory patterns.