Tissue Graft Insertion
Tissue Graft Insertion - Related Videos
13:04
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Cited by 13 •
2015
Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.
09:30
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
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Cited by 44 •
2016
The protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.
03:42
Solid Phase Microextraction Sampling: A Probe-based Technique for Sample Extraction from Graft Tissues
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2023
In this video, we demonstrate SPME-probe-based extraction of metabolites from a pre-harvested donor kidney. The extracted metabolites can be used to perform metabolomic and lipidomic analysis in order to assess the organ quality for transplantation.
11:17
Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring
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Cited by 9 •
2015
A step-by-step protocol for the inter-positional placement of Tissue Engineered Vessels (TEVs) into the carotid artery of a sheep using end-to-end anastomosis and real-time digital assessment in vivo until animal sacrifice.
12:39
Implantation of Inferior Vena Cava Interposition Graft in Mouse Model
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Cited by 24 •
2014
To improve our knowledge of cellular and molecular neotissue formation, a murine model of the TEVG was recently developed. The grafts were implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. This model achieves similar results to those achieved in our clinical investigation, but over a far shortened time-course.
FAQs
Successful engraftment depends on a sequence of connected events. The implanted tissue must attach to the prepared recipient site, regain adequate vascular support through revascularization, and integrate with surrounding host tissue. Failure at any stage can reduce cell survival or functional restoration. In experimental models, these processes also influence how faithfully the graft reproduces tissue-level behavior.
Immune and inflammatory responses can influence whether implanted tissue remains viable and becomes established in the host. Their effects occur alongside attachment, revascularization, and integration rather than as isolated events. Consequently, graft performance reflects both the biological properties of the tissue and its interaction with the recipient environment, which is important when interpreting cancer research models.
The technique places cancer-related tissue interactions in a biologically relevant setting rather than examining cellular behavior alone. This allows researchers to investigate tumor growth, interactions between tumor and surrounding tissue, treatment responses, and disease progression. By preserving tissue-level relationships, the approach can help connect observations from cellular studies with behavior occurring in a more complex host environment.
A general workflow includes selecting viable tissue, preparing the recipient site, positioning the graft within that site, and supporting conditions that allow attachment, revascularization, and integration. The procedure is not complete when placement ends, because subsequent host responses influence engraftment. In cancer research, the resulting tissue model is then used to examine growth, interactions, progression, or treatment response.
Researchers can use the approach when they need to examine tumor behavior within tissue relationships that are not represented fully by cellular findings alone. Supported applications include studying tumor growth, tissue interactions, disease progression, and responses to treatment. These uses make the method relevant for evaluating how cancer-related processes behave in a biologically meaningful graft-host setting.
Tissue graft models can provide evidence about how a tumor or other relevant tissue behaves after placement in a host environment, including patterns of growth, progression, tissue interaction, and treatment response. Such observations help researchers assess therapeutic strategies at the tissue level and relate those findings to cellular results, improving the biological context for experimental interpretation.