Tmem Doorway
Tmem Doorway - Related Videos
09:42
Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis
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Cited by 20 •
2019
We describe two methods for assessing transient vascular permeability associated with tumor microenvironment of metastasis (TMEM) doorway function and cancer cell intravasation using intravenous injection of high-molecular weight (155 kDa) dextran in mice. The methods include intravital imaging in live animals and fixed tissue analysis using immunofluorescence.
07:05
Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer
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Cited by 6 •
2015
A Cartesian bioprinter was designed and fabricated to allow multi-material deposition in precise, reproducible geometries, while also allowing control of environmental factors. Utilizing the three-dimensional bioprinter, complex and viable constructs may be printed and easily reproduced.
06:49
Using Eye Movements to Evaluate the Cognitive Processes Involved in Text Comprehension
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Cited by 138 •
2014
The present article describes how to use eye tracking methodologies to study the cognitive processes involved in text comprehension. Descriptions of eye tracking equipment, how to develop experimental stimuli, and procedural recommendations are included. The information presented can be applied to most any study using verbal stimuli.
07:33
Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
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Cited by 10 •
2011
An efficient method to gain insights into visualizing the paracrine-derived ROS induction of endothelial Ca2+ signaling is described. This method takes advantage of measuring paracrine derived ROS triggered Ca2+ mobilization in vascular endothelial cells in a co-culture model.
09:14
Low-stress Route Learning Using the Lashley III Maze in Mice
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Cited by 25 •
2010
The Lashley III maze is a route-learning task that does not rely on aversive stimuli or visual cues. It is thus a highly attractive option for evaluating learning and memory, especially in aging mice or otherwise where stress is a consideration.
FAQs
Macrophage-derived signals increase the permeability of nearby endothelial cells, making the vascular lining more permissive to tumor-cell passage. This change is important because it links immune-cell activity to a physical step in dissemination. In cancer research, examining this signaling relationship helps clarify how local communication within the tumor microenvironment can facilitate entry into the bloodstream.
The combined interaction of tumor cells, macrophages, and endothelial cells creates a coordinated interface rather than an isolated cell effect. Macrophages contribute permeability-related signals, endothelial cells provide the vascular boundary, and direct contact with tumor cells supports intravasation. Studying the full multicellular interaction therefore reveals mechanisms that may be missed when each cell type is considered separately.
Abundance describes how many TMEM doorways are present, whereas activity reflects whether their cellular interactions are functionally promoting tumor-cell trafficking. This distinction matters because structural presence and metastatic behavior are not identical measurements. Considering both features can give cancer researchers a more informative basis for assessing metastatic risk and investigating the local processes associated with dissemination.
Researchers examine TMEM doorways by focusing on their abundance, activity, and the interactions among the participating tumor, macrophage, and endothelial cells. This approach connects cellular organization with processes such as endothelial permeability and tumor-cell intravasation. The resulting observations support studies of how the tumor microenvironment contributes to metastatic spread without treating dissemination as a tumor-cell-only process.
Assessment of TMEM doorway abundance and activity can support evaluation of a tumor's metastatic risk. These features provide information about whether the local microenvironment contains sites associated with tumor-cell entry into the bloodstream. In cancer research, such measurements help relate multicellular tumor organization to dissemination and can guide further investigation of the mechanisms underlying metastatic potential.
TMEM doorway research can guide therapies designed to disrupt the cellular interactions that support tumor-cell trafficking. Potential investigative targets identified in the source include macrophage function, endothelial remodeling, and the movement of tumor cells into the circulation. By focusing on these local processes, researchers can explore strategies aimed at limiting dissemination rather than addressing tumor growth alone.