Human Hematopoietic Engraftment
Human Hematopoietic Engraftment - Related Videos
10:52
Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
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Cited by 1 •
2018
Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying biology.
14:22
Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
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Cited by 19 •
2011
An ex vivo protocol to generate mature human red blood cells from hematopoietic stem/progenitors is described. Additionally we describe an efficient lentiviral-delivery method to knockdown the transcription factor TAL1 in primary erythroid cells. The efficiency of lentivirus mediated gene delivery is demonstrated using GFP expressing viruses.
14:37
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
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Cited by 19 •
2017
Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.
02:26
A Technique to Generate a Human PBMC-Engrafted Humanized Xenograft Mouse Model
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2025
This video demonstrates an assay to generate a humanized xenograft mouse model. An immunocompromised mouse is injected with cyclophosphamide to cause immune cell depletion. Patient-derived tumor cells mixed with human peripheral blood mononuclear cells (PBMCs) in a hydrogel are subcutaneously injected into the mouse, forming a tumor with infiltrating T cells.
11:30
Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
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Cited by 43 •
2010
The colony forming cell (CFC) assay is an in vitro assay in which hematopoietic progenitors form colonies in a semi-solid medium. A combination of colony morphology, cell morphology, and flow cytometry are used to assess the ability of the progenitors to proliferate and differentiate along the different hematopoietic lineages.
FAQs
Persistence depends on coordinated movement into suitable blood-forming tissues, followed by homing to supportive bone marrow niches. Once located there, human hematopoietic stem and progenitor cells adhere to local microenvironments that help maintain their activity. These interactions are important because successful localization provides the setting required for continued function rather than only temporary cell presence.
Bone marrow niches provide local tissue environments that support transplanted human hematopoietic stem and progenitor cells. Their importance extends beyond physical lodging: niche interactions help sustain the cells and support ongoing blood-cell production. Studying these relationships allows researchers to examine how human cells respond to, and function within, a recipient’s tissue environment.
Self-renewal allows transplanted stem and progenitor cells to maintain the cell population over time, while differentiation generates multiple hematopoietic lineages. Together, these processes distinguish sustained biological activity from short-lived survival. Evaluating both properties helps determine whether transplanted cells retain stem cell potential and can contribute broadly to blood and immune cell development.
An engraftment assay can reveal whether human hematopoietic stem and progenitor cells establish sustained function in blood-forming tissues and contribute to multiple lineages. The resulting evidence supports assessment of stem cell potential, lineage development, and cellular interaction with the surrounding tissue environment. These measurements connect transplantation outcomes with developmental behavior in a living system.
Key outcomes include establishment within supportive tissues, sustained function, regeneration of blood cells, and development of immune cells and other hematopoietic lineages. Researchers can also examine how transplanted human cells interact with the recipient’s microenvironment. Together, these outcomes provide a broader picture of regeneration and lineage production than measuring cell presence alone.
In developmental biology, this approach provides a way to study how human stem and progenitor cells maintain potential, generate distinct blood lineages, and respond to tissue environments. It connects cellular behavior with the development of blood and immune systems. The resulting context supports investigation of human hematopoiesis in transplantation, blood disorders, and regenerative therapies.