Vascular Perfusion
Vascular Perfusion - Related Videos
06:05
DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum
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Cited by 3 •
2017
Using a lipophilic 1,1'-Dioctadecy-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) staining technique, Ambystoma mexicanum can undergo vascular perfusion to allow for easy visualization of the vasculature.
10:56
Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
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Cited by 4 •
2022
The protocol describes the surgical procurement and subsequent decellularization of vascularized porcine flaps by the perfusion of sodium dodecyl sulfate detergent through the flap vasculature in a customized perfusion bioreactor.
07:05
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
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Cited by 10 •
2018
The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.
07:19
Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
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Cited by 3 •
2021
Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
FAQs
Flow is driven by a pressure difference generated by the heart or an external pump, but vessel resistance determines how easily fluid moves through the network. Vessel diameter is especially important because narrowing restricts flow, whereas branching patterns distribute it among tissues. These interacting factors determine whether developing organs receive adequate circulation.
Endothelial permeability controls how readily fluid and dissolved substances pass between the vascular space and surrounding tissue. This property affects exchange while maintaining the boundary between blood or perfusion fluid and the tissue environment. In developmental studies, changes in permeability can therefore provide information about vessel maturation and integration with growing organs.
Branching patterns determine how flow is distributed across different regions of a tissue. A network must connect effectively with growing organs so that circulation reaches the areas being formed and maintained. Examining these patterns alongside pressure, resistance, and vessel diameter helps reveal how vascular networks mature and how abnormal organization may impair development.
An external pump can generate the pressure gradient needed to move blood or a blood-like fluid through tissue or an engineered vascular network. By providing controlled perfusion conditions, it supports examination of flow, exchange, and tissue function when the natural heart-driven circulation is unavailable or when researchers need a defined experimental system.
Perfusion-based imaging systems allow researchers to examine fluid movement and vascular organization while studying embryonic development or growing organs. The resulting observations can help assess whether vessels form connections, distribute fluid through tissue, and support functional integration. Such measurements link vascular structure with the circulation required during development.
Perfusion culture systems provide a way to evaluate engineered vascular networks under flowing conditions rather than examining structure alone. Researchers can assess whether the network supports fluid movement, exchange, and tissue function. These experiments also offer context for understanding how impaired circulation contributes to developmental abnormalities and disease.