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Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
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Bioengineering
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JoVE Journal Bioengineering
Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

DOI:
10.3791/64068-v

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10:56 min

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August 01, 2022

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, , ,
1Latner Thoracic Surgery Research Laboratories,Toronto General Hospital Research Institute, University Health Network, 2Institute of Laboratory Medicine and Pathobiology,University of Toronto, 3Division of Thoracic Surgery, Department of Surgery,University of Toronto, 4Division of Plastic and Reconstructive Surgery, Department of Surgery,University of Toronto

Chapters

  • 00:05Introduction
  • 00:57Perfusion Bioreactor Fabrication
  • 01:29Procurement of Porcine Flaps
  • 05:51Setup of the Decellularization System
  • 07:39Decellularization of Porcine Flaps and Evaluation of Decellularization
  • 09:06Results: Perfusion-Decellularization of Porcine Vascularized Flaps
  • 10:16Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes the surgical procurement and subsequent decellularization of vascularized porcine flaps by the perfusion of sodium dodecyl sulfate detergent through the flap vasculature in a customized perfusion bioreactor.

Tags

Keywords: PorcineVascularized FlapsPerfusion-decellularizationBioreactorReconstructive SurgeryOmental FlapTensor Fascia Lata FlapProcurementCannulationPerfusionDecellularizationRecellularization

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