Vascular Staining
Vascular Staining - Related Videos
00:06
Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
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Cited by 6 •
2019
Here, we present a protocol for immunofluorescence staining to observe the endothelial cells of the mouse aorta directly. This technique is useful when studying the cellular and molecular phenotype of endothelial cells in different flow patterns and in the development of atherosclerosis.
03:10
Von Kossa Staining of Aortic Vascular Smooth Muscle Cells: An In Vitro Technique for Assessment of Arterial Medial Layer Calcification
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2025
This video demonstrates a staining technique for assessing arterial medial calcification of the vascular smooth muscle cells in vitro. The von Kossa method employs a photochemical reaction to deposit metallic silver at the sites of calcification and impart a black coloration.
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
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Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
11:33
Microscopy and Staining: Gram, Capsule, and Endospore Staining
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2023
Source: Rhiannon M. LeVeque1, Natalia Martin1, Andrew J. Van Alst1, and Victor J. DiRita1 1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America Bacteria are diverse microorganisms found nearly everywhere on Earth. Many properties help distinguish them from each other, including but not limited to Gram-staining type, shape and arrangement, production of capsule, and formation of spores. To observe these properties, one...
07:32
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
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Cited by 7 •
2014
The rodent retina has long been recognized as an accessible window to the brain. In this technical paper we provide a protocol that employs the mouse model of oxygen-induced retinopathy to study the mechanisms that lead to failure of vascular regeneration within the central nervous system after ischemic injury. The described system can also be harnessed to explore strategies to promote regrowth of functional blood vessels within the retina and CNS.
FAQs
The signal comes from dyes or labeled probes binding to vessel-associated structures, creating contrast against surrounding tissue. This contrast can expose vessel walls, lumens, branching patterns, and the spatial distribution of vessels. Because these features become visible in images, researchers can examine organization at the tissue level rather than relying only on gross anatomical observations.
Perfusion and section-based staining provide different routes for exposing vascular structures to the labeling material. Perfusion can reveal vessels within intact tissue context, whereas applying a stain to sections targets structures exposed on the cut surface. The selected route therefore influences which vessel features are visible and how completely their organization can be assessed.
In plant material, contrasting stains produce visual differences between xylem and phloem, allowing these two vascular tissues to be located and compared within the same specimen. This distinction supports analysis of plant vascular organization and helps relate tissue arrangement to transport patterns across species, tissues, or environmental conditions.
Stained images can support measurements of vessel walls, lumens, branching, and vascular density. These features provide separate dimensions of organization: wall and lumen appearance describe local structure, while branching and density describe distribution across a tissue. Comparing those measurements helps identify differences among tissues, species, or environmental conditions.
A typical workflow selects animal tissue or plant material, applies the appropriate staining route, and examines the resulting preparation by imaging. Animal samples may receive staining through perfusion or on tissue sections, while plant samples may use contrasting stains to separate xylem and phloem. Researchers then measure visible vascular features for comparison.
The method is useful when environmental conditions may be associated with changes in vascular organization. Researchers can compare stained tissues across species, tissue types, or conditions and examine differences in vessel density, branching, or tissue identity. These observations connect microscopic structure with transport, development, tissue injury, and vascular responses to stress.