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Chapters
- 00:04Title
- 00:35Perfusion, Dissection, Longitudinally Opening of the Mouse Aorta
- 02:47Pretreatment, Immunostaining and Mounting of the Aorta
- 04:37Results: Representative En Face Staining Images
- 05:54Conclusion
Here, we present a protocol for immunofluorescence staining to observe the endothelial cells of the mouse aorta directly. This technique is useful when studying the cellular and molecular phenotype of endothelial cells in different flow patterns and in the development of atherosclerosis.
