B-scan Alignment
B-scan Alignment - Related Videos
09:45
Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies
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Cited by 6 •
2018
Here we present a protocol for wide-area scanning probe nanolithography enabled by the iterative alignment of probe arrays, as well as the utilization of lithographic patterns for cell-surface interaction studies.
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
02:28
Leaky Scanning
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2020
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
01:17
Design Example: Alignment of a Road Line Using GIS
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2025
The alignment of a road line using Geographic Information Systems (GIS) is a critical process in civil engineering, combining advanced technology with practical decision-making. This methodology begins with the collection of geospatial data, including information on land cover, geomorphology, drainage patterns, slope, and contour details. Such data is typically acquired through satellite imagery and GIS tools, offering a comprehensive understanding of the terrain.Once the data is gathered, it...
14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
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Cited by 14 •
2011
The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
FAQs
Returning sound echoes are converted into brightness values, creating the visual structure of the scan. Alignment therefore depends not only on probe placement but also on how consistently the resulting brightness pattern represents the target anatomy. Maintaining comparable orientation helps distinguish genuine tissue changes from differences caused by viewing the anatomy from another position.
The scan angle determines which cross-sectional view of the target anatomy appears in the image. Small changes in angle can alter the visible arrangement of landmarks and tissue features, making images harder to compare. Controlled angle adjustments help reproduce a consistent view, which is important when monitoring changes across time or experimental conditions.
Anatomical landmarks provide reference points for judging whether the imaging plane matches the intended target. When these landmarks appear in a consistent arrangement, researchers can more confidently compare one scan with another. This reference-based approach supports reliable assessment of tissue changes, particularly when inflammation, edema, or localized infection may modify the appearance of the region.
Misalignment can change the cross-sectional view and make a tissue region appear different even when its underlying condition has not changed. That variation may complicate measurements or comparisons involving inflammation, edema, or localized infection. Consistent probe position, landmarks, and scan angle reduce this source of variability and strengthen interpretation of disease progression or treatment response.
Begin by positioning the probe over the target anatomy, then adjust its orientation and scan angle until the intended landmarks and imaging plane are represented. The resulting view should be checked for consistency before images are compared or measurements are made. Repeating these controlled placement and adjustment steps supports more dependable longitudinal observations.
This alignment approach is useful when researchers need to follow tissue changes associated with inflammation, edema, or localized infection. Consistent scans can support comparisons during disease progression studies and when evaluating experimental treatments. By helping preserve a comparable cross-sectional view, the technique contributes to more reliable interpretation of changes between observations.