Microscopy Examination
Microscopy Examination - Related Videos
08:35
Examination of Drosophila Larval Tracheal Terminal Cells by Light Microscopy
0 Views •
Cited by 11 •
2013
Here, we present a method for light microscopy analysis of tracheal terminal cells in Drosophila larvae. This method allows for quick examination of branch and lumen morphology in whole animals and would be useful for analysis of individual mutants or screens for mutations affecting terminal cell development.
09:37
Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
0 Views •
Cited by 71 •
2014
More than half of proteins are small proteins (molecular mass <200 kDa) that are challenging for both electron microscope imaging and three-dimensional reconstructions. Optimized negative staining is a robust and high-throughput protocol to obtain high contrast and relatively high resolution (~1 nm) images of small asymmetric proteins or complexes under different physiological conditions.
07:44
Ophthalmoscopic Examination
0 Views •
2023
Source: Richard Glickman-Simon, MD, Assistant Professor, Department of Public Health and Community Medicine, Tufts University School of Medicine, MA The simplest ophthalmoscopes consist of an aperture to look through, a diopter indicator, and a disc for selecting lenses. The ophthalmoscope is primarily used to examine the fundus, or the inner wall of the posterior eye, which consists of the choroid, retina, fovea, macula, optic disc, and retinal vessels (Figure 1). The spherical eyeball...
35:03
Mental Status Examination
0 Views •
2023
Source: Carmen Black, Matthew Goldenberg, and Jaideep Talwalkar; Yale School of Medicine The mental status exam is a clinical evaluation of emotional, perceptual, and cognitive functioning that spans both the fields of psychiatry and neurology. Developing skill in the psychiatric portions of the examination are important to describe salient aspects of the patient's mental state that may be observed during routine history and/or physical exam. Proficiency in the cognitive portions of this...
20:06
The Comprehensive Infant Examination
0 Views •
2023
Source: Heather Collette and Jaideep Talwalkar; Yale School of Medicine The newborn exam is important in establishing a baby's baseline health status as they enter the world. Newborn providers have the unique opportunity to evaluate a patient for the very first time. They are also tasked with identifying congenital anomalies that require early intervention to promote a long and healthy life for the child. Many newborn anomalies, such as heart defects, are noted on prenatal ultrasound. However,...
FAQs
Optical instruments use illumination, whereas electron-based instruments use an electron beam to interact with the specimen. In both cases, lenses and detectors convert specimen-related differences into an image, but the selected instrument determines how the sample is examined and which structural features become accessible. This distinction helps researchers match microscopy examination to the cells, tissues, or microorganisms under study.
Preparation can determine which specimen features remain preserved and which structural differences become emphasized in the resulting image. A preparation approach therefore affects not only image quality but also the biological interpretation of what is observed. In infection and immunology studies, this is important when examining microbial morphology, tissue changes, or the spatial relationship between pathogens and host cells.
Illumination or an electron beam interacts with the specimen and produces differences that carry structural information. Lenses help magnify and resolve those differences, while detectors collect the resulting signal for conversion into a visible image. Contrast methods further distinguish specimen features, allowing researchers to interpret boundaries, morphology, localization, or tissue alterations that would otherwise be difficult to see.
A microscopy examination begins with preparing the specimen so relevant structures are preserved or highlighted. The sample is then examined with an optical or electron-based instrument, using illumination or an electron beam, followed by lens and detector systems that generate an image. Researchers interpret the resulting structural patterns in relation to the biological question, such as infection or immune-cell interaction.
It is useful when investigators need visual evidence of microbial morphology, pathogen location, or changes within infected tissues. The resulting images can show where a pathogen is situated relative to surrounding structures and can support analysis of tissue responses. These observations contribute to infection research by connecting visible structural changes with the distribution of microorganisms in biological samples.
Microscopy examination enables researchers to visualize interactions between immune cells and pathogens and to assess structural changes in tissues. This spatial information complements broader analysis by showing where cellular or microbial features occur within a specimen. In immunology and infection research, such images help characterize host responses alongside pathogen localization and the morphology of affected cells or tissues.