Pbs Colon Flushing
Pbs Colon Flushing - Related Videos
07:08
Assessing and Flushing a Peripheral Intravenous Line
0 Views •
2023
Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT After peripheral intravenous (IV) access is initiated, it is important to assess and maintain the IV catheter according to institutional policies and nursing standards of practice. The regular assessment of the insertion site and the surrounding areas for signs of complications is necessary to prevent IV catheter complications, including infiltration, phlebitis, infection, extravasation, or...
03:20
Electrophysiological Recording from Colonic Afferent Nerves in an Ex Vivo Rat Colon
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2025
This video demonstrates a method to record the activity of colonic afferent nerves in an ex-vivo rat colon, cannulated at both ends and infused with saline to increase intraluminal pressure. As pressure increases, the nerve signal rises, indicating its stimulation.
09:12
Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
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Cited by 27 •
2014
Colonization of the murine nasopharynx with Streptococcus pneumoniae and the subsequent extraction of adherent or recruited cells is described. This technique involves flushing the nasopharynx and collection of the fluid through the nares and is adaptable for various readouts, including differential cell quantification and analysis of mRNA expression in situ.
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
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Cited by 43 •
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The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.
10:19
A Murine Model of Group B Streptococcus Vaginal Colonization
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Cited by 64 •
2016
The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.
FAQs
PBS contributes both chemical stability and physical recovery. Its buffered composition helps keep collected material in a suitable pH environment, while its near-isotonic character helps preserve sample integrity during rinsing. The fluid also mechanically dislodges material associated with the colonic lumen, allowing mucus, microbes, immune cells, and soluble molecules to enter the collected sample for downstream analysis.
The recovered material can contain several complementary indicators of intestinal status, including luminal microbes, mucus-associated material, immune cells, and soluble molecules. Examining these components together can help investigators evaluate microbial burden, inflammatory changes, and local immune activity rather than relying on a single measurement. This supports broader interpretation of host-microbe and host-pathogen interactions in the colon.
Standardized rinsing provides a consistent sampling approach across experimental conditions. Greater consistency helps researchers distinguish biological differences from variation introduced during collection, strengthening comparisons of luminal inflammation, microbial burden, and immune responses. The resulting measurements can also be related to tissue-level or systemic immune outcomes, helping clarify how local changes in the gut environment correspond with broader host responses.
A colon flushing sample provides information about the intestinal lumen, including its microbial, cellular, and soluble contents. Tissue or systemic measurements address different levels of the immune response, so combining them can reveal connections between the gut environment and outcomes elsewhere in the host. This layered approach is useful for studying inflammation and host-pathogen interactions in immunology and infection research.
The workflow centers on rinsing the large intestine with PBS and collecting the material displaced from the lumen. The recovered sample is then available for analyses aimed at assessing intestinal inflammation, microbial burden, immune responses, or soluble molecules. Maintaining a consistent collection approach across experimental groups is important because the method is intended to support meaningful comparisons.
Researchers may select this approach when they need a standardized sample of colonic luminal contents rather than information from intestinal tissue alone. It is relevant to experiments examining inflammation, microbial burden, immune activity, or host-pathogen interactions. Comparing recovered material across conditions can help identify how changes within the gut relate to tissue-level findings or systemic immune outcomes.