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Immunology and Infection
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Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
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Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

DOI:
10.3791/50490-v

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09:12 min

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January 17, 2014

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, , ,
1Department of Pathology and Molecule Medicine,McMaster University

Chapters

  • 00:00Title
  • 01:55Murine Intranasal Colonization
  • 03:58Nasal Lavage
  • 06:46Quantification of Nasopharyngeal Bacterial Load
  • 08:41Conclusion

Summary

Automatic Translation

Automatic Translation

Colonization of the murine nasopharynx with Streptococcus pneumoniae and the subsequent extraction of adherent or recruited cells is described. This technique involves flushing the nasopharynx and collection of the fluid through the nares and is adaptable for various readouts, including differential cell quantification and analysis of mRNA expression in situ.

Tags

Keywords: Streptococcus PneumoniaeNasopharyngeal ColonizationIntranasalInflammatory ResponseImmune ResponseMouse ModelPneumoniaBacteremiaMeningitisResident MacrophagesLymphocytesBacterial Density

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