In Vitro Expansion
In Vitro Expansion - Related Videos
09:42
Analysis of Thermal Expansion via Dilatometry
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2023
Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application. For instance, in a common liquid thermometer, a material with a high thermal expansion would be...
09:45
Ultrastructural Expansion Microscopy in Three In Vitro Life Cycle Stages of Trypanosoma cruzi
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Cited by 4 •
2023
This study shows a detailed protocol to perform ultrastructure expansion microscopy in three in vitro life cycle stages of Trypanosoma cruzi, the pathogen responsible for Chagas disease. We include the optimized technique for cytoskeletal proteins and pan-proteome labeling.
12:06
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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Cited by 22 •
2011
Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.
08:37
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
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Cited by 5 •
2021
We describe a rapid and robust protocol to enrich invariant natural killer T (iNKT) cells from mouse spleen and expand them in vitro to suitable numbers for in vitro and in vivo studies.
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Cited by 21 •
2020
This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
FAQs
Cell viability and desired characteristics depend on coordinated control of nutrients, temperature, gas conditions, and growth signals. These inputs create the environment needed for cells or tissues to multiply while retaining properties relevant to the intended use. If conditions are poorly controlled, expansion may produce populations that are less consistent or less suitable for research and medical applications.
Passaging allows multiplication to continue as populations grow and provides repeated opportunities to maintain the culture under suitable conditions. Researchers subculture the expanding population and then monitor growth before repeating the cycle. This approach helps generate a larger population while supporting ongoing assessment of viability and the characteristics required for downstream research or medical use.
Sterile culture conditions protect the expansion process from contamination, while regular monitoring shows whether the population remains viable and develops as expected. These checks matter because the goal is not simply to increase cell number. Researchers also need a consistent population with desired characteristics, particularly when expanded cells will support medical research or clinically relevant applications.
A practical workflow begins by maintaining cells or tissues in a controlled sterile environment with suitable nutrients, temperature, gas conditions, and growth signals. Researchers then monitor growth, viability, and contamination as the population increases. When multiplication requires continuation, they perform subculturing or passaging and repeat the cycle until enough cells are available for the intended use.
In medical development, expanded cell populations can support cell-based therapies, regenerative medicine, and tissue engineering. Maintaining viability and desired characteristics is important because these applications depend on populations that remain suitable for their intended use. Monitoring during culture helps connect increased cell numbers with consistency and clinical relevance, rather than treating expansion alone as the final outcome.
Beyond therapeutic development, expanded cells provide material for disease modeling and drug evaluation. Increasing the available population can support studies that require cells or tissues to be maintained under controlled culture conditions. Researchers still need to monitor viability, growth, contamination, and desired characteristics so that experimental results reflect a consistent biological model or evaluation system.