Intrahepatic Xenograft
Intrahepatic Xenograft - Related Videos
10:35
Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
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Cited by 15 •
2013
Human tumor xenografts in immunodeficient mice are valuable tools to study cancer biology. Specific protocols to generate subcutaneous and intrahepatic xenografts from human hepatocellular carcinoma cells or tumor fragments are described. Liver regeneration induced by partial hepatectomy in recipient mice is presented as a strategy to facilitate intrahepatic engraftment.
03:45
Right Hemihepatectomy by Suprahilar Intrahepatic Transection of the Right Hemipedicle using a Vascular Stapler
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Cited by 1 •
2010
This video describes a right hemihepatectomy with intrahepatic transection of the right hemipedicle leaving the hepatoduodenal ligament completely untouched.
03:21
Zebrafish Patient-Derived Xenograft (zPDX): A Cancer Model
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2023
This video describes the technique to create a zebrafish patient derived xenograft model. In the sample protocol, we assess the efficiency of the drugs Gemcitabine and Navitoclax on the pancreatic tumor xenograft model.
07:55
The Influence of Liver Resection on Intrahepatic Tumor Growth
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Cited by 6 •
2016
A high incidence of tumor recurrence after resection of liver metastases remains an unsolved problem. The illustrated mouse model may be useful to investigate the reasons for such recurrences. It combines a liver resection model with intrahepatic tumor cell injection for the first time.
11:44
Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
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Cited by 40 •
2010
Cancer stem cells (CSCs) have been identified in a number of malignancies. In this protocol we describe a flow cytometric method utilizing aldehyde dehydrogenase activity and CD44 and CD24 expression to isolate CSCs from human pancreatic adenocarcinoma xenografts. These viable cells can then be used in functional and analytical studies.
FAQs
The graft’s behavior depends on more than the implanted cells or tissue. Local blood supply can affect survival and growth, while interactions with liver stromal components shape how the graft develops within hepatic tissue. These microenvironmental influences also affect treatment response, making the model useful for examining disease behavior under conditions that include the surrounding liver environment.
Host immune status is a major variable because it can influence whether implanted material survives and how it grows. It may also alter the observed response to treatment. Consequently, findings from an intrahepatic xenograft must be considered in relation to the host environment, rather than attributed solely to the properties of the implanted cells, tissue, or tumor.
A cell-based experiment examines biological behavior outside a living organism, whereas an intrahepatic xenograft places the material within liver tissue. This setting incorporates local blood supply, stromal interactions, and host immune status. The resulting in vivo context can reveal effects on survival, growth, and treatment response that are not represented by isolated cell-based systems.
These models support investigation of liver cancer, metastasis, and other hepatic diseases. Because the graft develops within the liver, researchers can examine how disease-related material behaves in a hepatic setting, including its survival and growth. This makes the approach relevant when the research question depends on interactions between abnormal tissue and the liver environment.
Researchers can use the model to examine how implanted tumors or other disease-related material responds to anticancer drugs and therapeutic strategies. Outcomes may include changes in graft survival, growth, or treatment response. Testing these effects in a living hepatic environment helps connect treatment observations from experimental systems with questions relevant to clinical research.
Intrahepatic xenografts combine an implanted graft with the biological context of a living organism. This allows researchers to study human biology, disease behavior, and therapeutic responses while accounting for hepatic conditions that cell-based experiments do not reproduce. Their findings can therefore inform the transition from laboratory investigation toward clinical research without replacing clinical evaluation.