Leukocyte Rolling Quantification
Leukocyte Rolling Quantification - Related Videos
14:16
Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
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Cited by 32 •
2009
This report provides a visual depiction of parallel-plate flow chamber analysis for studying leukocyte endothelial interactions under physiologic shear stress. This method is particularly useful for investigating the role of endothelial (E)-selectin and leukocyte E-selectin ligands that trigger leukocyte rolling on endothelial cell surfaces.
05:12
Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
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Cited by 11 •
2015
This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.
10:16
Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls
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Cited by 6 •
2018
This manuscript describes the use of transgenic reporter mice and different administration routes of fluorescent dyes in angiotensin II-induced hypertension using intravital video microscopy of blood vessels to evaluate the activation of immune cells and their ability to roll and adhere to the endothelium.
05:54
Quantification of Diabetes-induced Adherent Leukocytes in Retinal Vasculature
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2025
We demonstrate a method to label the walls of the retinal vasculature and adherent leukocytes. These adherent leukocytes can then be counted under a fluorescence microscope as a parameter of inflammation or the response of that inflammation to therapies.
07:42
Non-invasive Imaging of Leukocyte Homing and Migration in vivo
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Cited by 8 •
2010
Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.
FAQs
Blood-flow shear does not simply move leukocytes past the vessel wall; it tests the timing of repeated selectin-ligand contacts. Each contact briefly tethers a cell, then releases it, producing a sequence of slowing and renewed motion. Quantifying these interactions reveals how effectively endothelial selectins support early leukocyte recruitment under dynamic vascular conditions.
Rolling velocity and rolling frequency describe different features of the response. Velocity indicates how quickly an interacting leukocyte travels along the endothelium, whereas frequency reflects how often cells enter the rolling population. Rolling duration adds the persistence of individual interactions, and the proportion of interacting cells estimates how broadly the vascular surface engages circulating leukocytes.
Changes in endothelial activation can alter the measured rolling pattern because activated endothelium participates in leukocyte recruitment. Comparing rolling velocity, duration, frequency, and interacting-cell proportion therefore provides a multidimensional view rather than relying on one value. The combined profile can indicate whether a condition changes how many cells interact, how long they remain engaged, or how rapidly they move.
Leukocyte Rolling Quantification can be performed with live-cell imaging or flow-based assays that preserve the influence of blood-flow shear. A study records leukocyte-endothelium interactions, identifies rolling cells, and extracts measures such as velocity, duration, frequency, and interacting-cell proportion. Using more than one metric helps describe both individual cell behavior and the overall extent of vascular engagement.
Live-cell imaging is useful when researchers need to observe individual leukocytes as they tether, move, and release along the endothelial surface. Flow-based assays offer a complementary way to examine interactions under blood-flow conditions. The choice depends on whether the experiment emphasizes cell-by-cell dynamics, population-level interaction patterns, or both, while retaining rolling behavior as the measured outcome.
These measurements help connect vascular behavior with inflammatory and immune processes. Investigators can compare responses in studies of vascular disease, infection, and immune disorders, then assess whether therapies targeting leukocyte recruitment change rolling behavior. Results may reveal differences in endothelial activation, leukocyte adhesion, or the extent of cellular interaction with the vascular surface.