Single-lumen Tubes
Single-lumen Tubes - Related Videos
10:30
The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging
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Cited by 6 •
2017
The C. elegans excretory canal is a unique single-cell model for the visual in vivo analysis of de novo polarized membrane biogenesis. This protocol describes a combination of standard genetic/RNAi and imaging approaches, adaptable for the identification and characterization of molecules directing unicellular tubulogenesis, and apical membrane and lumen biogenesis.
04:32
Chip-in-a-Tube-Based Digital PCR for Quantification of Single Nucleotide Variants
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2025
This video demonstrates the utility of digital PCR in a chip-in-a-tube format for the detection of single nucleotide variations in DNA samples. A single PCR reaction is partitioned into chambers that act as independent PCR reactions, and the detection of fluorescence signals from the amplified targets in the chambers is used to compute the frequency of the variant allele in the sample.
02:34
Quantification of Group B Streptococcus Load in the Mouse Vaginal Lumen via Swabbing and Chromogenic Culture
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2026
Source: Patras, K. A. & Doran, K. S. A Murine Model of Group B Streptococcus Vaginal Colonization. J. Vis. Exp. (2016)This video demonstrates the quantification of vaginal Group B Streptococcus (GBS) load in a mouse model using swabbing and chromogenic culture. It outlines the steps involved in sample collection, plating, and colony counting to quantify GBS load in the mouse vaginal lumen.
03:21
Localized Delivery of Bacteria into the Intestinal Lumen of a Zebrafish Larva Using Microinjection
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2025
Source: Li, J. et al. Development of a Larval Zebrafish Infection Model for Clostridioides difficile. J. Vis. Exp. (2020)This video demonstrates the microinjection-based delivery of fluorescently labeled Clostridioides difficile into the intestinal lumen of an anesthetized zebrafish larva. It outlines the steps involved in larval preparation, bacterial injection, and fluorescence microscopy–based confirmation of bacterial localization within the intestine.
12:15
The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
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Cited by 13 •
2017
The transparent C. elegans intestine can serve as an "in vivo tissue chamber" for studying apicobasal membrane and lumen biogenesis at the single-cell and subcellular level during multicellular tubulogenesis. This protocol describes how to combine standard labeling, loss-of-function genetic/RNAi and microscopic approaches to dissect these processes on a molecular level.
FAQs
Performance depends on matching the tube’s diameter and flexibility to the procedure and anatomy. Diameter influences the passage available for gas or fluid, while flexibility affects how the device can be positioned. The external connection must also suit the intended equipment, such as a ventilator or collection system, so the passage supports controlled delivery, withdrawal, drainage, or sampling.
When present, a cuff or another securing feature helps maintain the tube’s position after placement. A cuff can also limit leakage around the tube, which is particularly relevant when the passage is used for airway management and mechanical ventilation. These features support more controlled movement of gases or fluids, but their usefulness depends on the design and clinical application.
The same basic passage can serve different purposes because its effect depends on where it is positioned and what it connects to. Placement in an airway supports gas movement, whereas positioning for access to fluid or a collection site can enable drainage or specimen withdrawal. Consequently, the intended direction of movement and the target anatomy guide tube selection.
Selection should match the tube’s material and dimensions to the procedure, the patient’s anatomy, and the required flow. The clinician also needs to consider whether the tube will deliver gases or fluids, remove material, or collect a specimen. Compatibility with external equipment and the need for securing or leakage-limiting features further shape the appropriate choice.
Single-lumen endotracheal tubes establish airway access for ventilation and airflow management. Their dimensions and flexibility must suit the relevant anatomy, while a cuff or other securing feature may help maintain position and limit leakage when included in the design. Connection to mechanical ventilation equipment allows gases to move through the tube in support of airway management.
Related single-lumen designs support several access functions beyond airway management, including suction, drainage, infusion, and specimen collection. The required direction of movement determines whether the tube delivers material or permits withdrawal. Choosing dimensions, material, and compatible external equipment for that specific task helps align the device with the intended clinical procedure and the needed flow.