Single Operator Technique

Single operator technique is a medical procedural approach in which one clinician performs the key steps of an intervention, coordinating visualization, instrument handling, and patient monitoring within a streamlined workflow. The operator advances and manipulates the required device while continuously assessing anatomy, procedural progress, and patient status, often with support staff available for preparation or assistance. This approach can simplify team coordination, clarify responsibility, and standardize procedural training when the technique and clinical setting are appropriate. Studying single operator technique helps clinicians evaluate workflow efficiency, technical performance, safety considerations, and its potential role in modern procedural medicine.

Single Operator Technique - Related Videos

Research

JoVE Journal - Bioengineering
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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

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Cited by 75 •

2013

In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.

Education

JoVE Science Education - Basic Biology

Operating the Glovebox

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2023

Robert M Rioux, Ajay Sathe, Zhifeng Chen, Pennsylvania State University, University Park, PA A glovebox is an isolated enclosure that is designed to maintain an inert atmosphere. The manipulation of chemicals or apparatus is done via the use of gloves, which allow operation while still maintaining an inert atmosphere. A positive pressure is utilized to compensate for any passive leaks. Objects can be transferred in and out of the box using an antechamber that acts as an airlock between the box...

The Modified Single-working Portal Technique Using Lasso-loop Stitch with Needle for Arthroscopic Subscapularis Repair

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2025

We propose a modified arthroscopic technique of suturing the subscapular using a needle-through-line Lasso-loop through a unilateral anterior portal.

Operant Sensation Seeking in the Mouse

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Cited by 27 •

2010

In this protocol we describe a method of operant learning using sensory stimuli as a reinforcer in the mouse. It requires no prior training or food restriction, and it allows the study of motivated behavior without the use of a pharmacological or natural reinforcer such as food.

Single Cell Sampling Using Micromanipulation: A Technique to Isolate Single Cells from Cancer Cell Suspension

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2023

This video describes the technique for isolating single cells from a pool of target cells to carry out downstream analysis. In this method, a micromanipulator fitted to a microscope is used for high-precision isolation of a single target cell.

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