Vascular Disease Model
Vascular Disease Model - Related Videos
08:50
Assessment of Vascular Function in Patients With Chronic Kidney Disease
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Cited by 25 •
2014
The degree of vascular dysfunction and contributing physiological mechanisms can be assessed in patients with chronic kidney disease by measuring brachial artery flow-mediated dilation, aortic pulse-wave velocity, and vascular endothelial cell protein expression.
09:06
Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease
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Cited by 7 •
2012
We describe a technique in which a section of the abdominal aorta from a mouse is transplanted orthotopically to just below the renal arteries in an allogeneic or syngeneic recipient. This technique can be useful in studies in which transplantation of large arteries of uniform size is deemed advantageous.
09:23
Shunt Surgery, Right Heart Catheterization, and Vascular Morphometry in a Rat Model for Flow-induced Pulmonary Arterial Hypertension
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Cited by 15 •
2017
This protocol describes a surgical procedure to create a model for flow-induced pulmonary arterial hypertension (PAH) in rats and the procedures to analyze the principle hemodynamic and histological end-points in this model.
07:56
A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo
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Cited by 13 •
2014
Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.
07:41
A Model of Reverse Vascular Remodeling in Pulmonary Hypertension Due to Left Heart Disease by Aortic Debanding in Rats
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Cited by 2 •
2022
The present protocol describes a surgical procedure to remove ascending-aortic banding in a rat model of pulmonary hypertension due to left heart disease. This technique studies endogenous mechanisms of reverse remodeling in the pulmonary circulation and the right heart, thus informing strategies to reverse pulmonary hypertension and/or right ventricular dysfunction.
FAQs
These systems connect molecular and cellular changes with impaired blood flow. By examining endothelial dysfunction, inflammation, plaque formation, thrombosis, or abnormal vessel growth under controlled conditions, researchers can investigate how vascular disturbances develop and identify biological changes associated with disease. This mechanistic link supports the search for therapeutic targets rather than focusing only on clinical outcomes.
The selected system determines which level of vascular disease researchers can examine. Cultured endothelial cells provide a cellular setting, engineered tissues offer a more organized vascular environment, animal systems represent disease within an organism, and patient-derived samples retain features associated with individual patients. Together, these options provide complementary perspectives on vascular pathology.
Researchers can select a model according to the vascular process central to their question. Relevant features include endothelial dysfunction, inflammation, plaque formation, thrombosis, and abnormal vessel growth. The same general modeling strategy can therefore support studies of atherosclerosis, hypertension, aneurysms, and ischemic injury while focusing on different biological changes linked to impaired blood flow.
A general workflow starts by selecting an experimental system that represents the vascular condition or process of interest. Researchers then examine disease-related changes under controlled conditions and use the system to assess a candidate treatment. Measuring both disease-relevant effects and potential safety concerns allows the model to contribute to efficacy and drug-safety evaluation.
Vascular disease models allow investigators to examine disease mechanisms while testing how potential treatments affect those mechanisms. Observed changes can reveal biological processes suitable for therapeutic targeting, and treatment experiments can provide evidence about efficacy and safety. This combined use helps connect mechanistic findings with decisions about whether a therapy warrants further investigation.
Patient-derived samples can preserve characteristics associated with individual patients, adding a clinically relevant perspective to vascular research. When used alongside cultured cells, engineered tissues, or animal systems, they can help investigators compare disease-related features across experimental settings. This supports the broader goal of developing more personalized approaches to vascular care and treatment evaluation.