Ca1 Subiculum Recordings
Ca1 Subiculum Recordings - Related Videos
03:10
Recording CA3-CA1 Synaptic Responses in a Rat Hippocampal Slice
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2025
This video demonstrates a method to record CA3-CA1 synaptic responses in a rat hippocampal slice. It outlines the steps involved in placing the stimulating and recording electrodes on the hippocampal slice and measuring the field excitatory postsynaptic potential (fEPSP) to assess synaptic response strength.
11:37
Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
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Cited by 4 •
2017
Here, we present a protocol for recording rhythmic neuronal network theta and gamma oscillations from an isolated whole hippocampal preparation. We describe the experimental steps from extraction of the hippocampus to details of field, unitary and whole-cell patch clamp recordings as well as optogenetic pacing of the theta rhythm.
14:27
Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording
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Cited by 11 •
2019
We used recording and stimulation electrodes in longitudinal hippocampal brain slices and longitudinally positioned recording and stimulation electrodes in the dorsal hippocampus in vivo to evoke extracellular postsynaptic potentials and demonstrate long-term synaptic plasticity along the longitudinal interlamellar CA1.
02:58
Generating Local Cornu Ammonis 1 (CA1) Gamma Oscillations Using Tetanic Stimulations in a Hippocampal Brain Slice
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2025
This video demonstrates the procedure of inducing local CA1 gamma oscillations in a CA1 hippocampal mouse brain slice using tetanic simulations. This method is important to understand the neuronal network activity.
08:02
Generation of Local CA1 γ Oscillations by Tetanic Stimulation
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Cited by 2 •
2015
Oscillations are fundamental network properties and are modulated by disease and drugs. Studying brain-slice oscillations allows characterization of isolated networks under controlled conditions. Protocols are provided for the preparation of acute brain slices for evoking CA1 γ oscillations.
FAQs
These recording signals represent different levels of neural activity. Membrane-voltage measurements track changes within individual neurons, whereas synaptic-current measurements indicate electrical input received through synaptic connections. Extracellular field potentials capture voltage changes produced by groups of nearby cells. Comparing these signals helps separate cellular excitability from synaptic transmission and larger-scale circuit activity.
Spontaneous recordings show neural activity occurring without an imposed input, providing a view of ongoing circuit behavior. Controlled stimulation adds a defined perturbation, allowing researchers to examine how CA1 and the subiculum respond to an input. Together, these conditions help distinguish baseline activity from changes associated with synaptic transmission, circuit integration, or altered excitability.
The subiculum serves as a principal output structure of the hippocampal CA1 region, so recordings from both areas can be interpreted in relation to information flow through the circuit. Examining activity across the pair helps researchers investigate how signals are processed within CA1 and transmitted onward, rather than viewing either region as an isolated source of neural activity.
Changes in membrane voltage or synaptic currents can indicate how readily neurons respond and how strongly they receive synaptic input. Responses recorded during controlled stimulation provide information about functional connections between circuit elements, while extracellular field potentials show coordinated activity across nearby populations. Evaluating these measures together supports analysis of both individual cellular responses and circuit-level communication.
A typical recording workflow uses electrodes to detect activity in CA1, the subiculum, or both, while measurements are collected either spontaneously or during controlled stimulation. Researchers then examine membrane voltage, synaptic currents, or extracellular field potentials according to the question being tested. The resulting signals are interpreted for excitability, synaptic transmission, connectivity, and information flow.
These recordings can be used to study how hippocampal circuits integrate synaptic inputs and transmit information to connected brain regions. They also support investigations of processes relevant to learning and memory by revealing activity and communication within the CA1-subiculum pathway. The approach is useful when the research question concerns cellular responses as well as circuit-level processing.
By measuring neural activity and responses to stimulation, researchers can examine whether circuit excitability, synaptic transmission, or information flow differs under conditions associated with network dysfunction. Comparing activity patterns and evoked responses across experimental conditions can help characterize abnormalities in hippocampal circuitry. This makes CA1-subiculum recordings relevant to studies of mechanisms underlying neurological disease.