Journal
/
Neuroscience
/
Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

DOI:
10.3791/55851-v

•

11:37 min

•

August 02, 2017

•

,
1Department of Psychiatry, Douglas Mental Health University Institute,McGill University

Chapters

  • 00:05Title
  • 00:54Isolating the Hippocampus
  • 03:47Set Up Fast Perfusion for Recording the Isolated Hippocampus
  • 04:34Extracellular Recording on In Vitro Hippocampal Theta Oscillations
  • 07:57Optogenetic Control in the Isolated Hippocampus
  • 09:11Results: Cell-type Specific Activity of Pyramidal Cell and PV Interneuron During Spontaneous Theta Oscillations in the Intact Hippocampal Preparation from a PV-TOM Mouse
  • 10:19Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for recording rhythmic neuronal network theta and gamma oscillations from an isolated whole hippocampal preparation. We describe the experimental steps from extraction of the hippocampus to details of field, unitary and whole-cell patch clamp recordings as well as optogenetic pacing of the theta rhythm.

Tags

Keywords: HippocampusTheta BandIn VitroSeptohippocampal CircuitField RecordingsUnitary RecordingsPatch-clamp RecordingsOptogenetic StimulationRhythmic Neuronal NetworkLearning And MemoryOscillations

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55851?article_type=v

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
check_url/55851?article_type=v

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury
check_url/55851?article_type=v

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
check_url/55851?article_type=v

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
check_url/55851?article_type=v

Neural Circuit Recording from an Intact Cockroach Nervous System
check_url/55851?article_type=v

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
check_url/55851?article_type=v

Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain
check_url/55851?article_type=v

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
check_url/55851?article_type=v

Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons
check_url/55851?article_type=v

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Read Article