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Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
Tuning in the Hippocampal Theta Band <em>In Vitro</em>: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

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11:37 min

August 02, 2017

DOI:

11:37 min
August 02, 2017

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Summary

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Here, we present a protocol for recording rhythmic neuronal network theta and gamma oscillations from an isolated whole hippocampal preparation. We describe the experimental steps from extraction of the hippocampus to details of field, unitary and whole-cell patch clamp recordings as well as optogenetic pacing of the theta rhythm.

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