Confocal Reflectance
Confocal Reflectance - Related Videos
06:07
Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network
0 Views •
2025
This method visualizes the native structure of collagen fibers in the vitreous body using confocal reflectance microscopy.
13:59
Reflection and Refraction
0 Views •
2023
Source: Derek Wilson, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA Light travels at different speeds depending on the material through which it is propagating. When light travels from one material to another, it will either slow down or speed up. In order to conserve energy and momentum, the light must change the direction in which it propagates. This bending of light is known as refraction. Some fraction of the...
10:22
Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
0 Views •
Cited by 5 •
2017
This protocol efficiently studies mammalian cell division in 3D collagen matrices by integrating synchronization of cell division, monitoring of division events in 3D matrices using live-cell imaging technique, time-resolved confocal reflection microscopy and quantitative imaging analysis.
02:57
The Sense of Self: Reflected Self-Appraisal and Social Comparison
0 Views •
2020
According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
0 Views •
Cited by 14 •
2011
The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
FAQs
The confocal pinhole limits detection to reflected or backscattered light originating near the focused point. Light from regions outside that focal plane is rejected, reducing background and improving image contrast. This selective detection also supports axial resolution, allowing researchers to distinguish structural features at different depths rather than combining signals from the entire specimen thickness.
Image contrast comes from differences in how specimen structures reflect or backscatter the focused illumination. Cellular and tissue components can therefore appear based on their optical properties rather than on attached fluorophores. This makes the method useful for examining neural morphology and organization when fluorescence labeling is undesirable or unavailable.
Point-by-point scanning coordinates illumination and detection at defined locations across the specimen. Combined with confocal rejection of out-of-focus light, this produces spatially resolved optical sections instead of an undifferentiated depth-integrated image. The resulting sections help reveal cellular and tissue morphology within neural samples while preserving information about structural organization.
A focused beam is directed to the neural specimen and scanned across it point by point. Reflected or backscattered light is collected from each location, while the confocal pinhole suppresses light from outside the focal region. The detected signal is then used to form high-resolution optical sections, enabling inspection of structure at selected depths.
Researchers may select this approach when they need to examine neural structure without fluorescence labeling, or when labels are unavailable or undesirable. It can be applied to living or fixed neural samples, supporting observations of cellular morphology, tissue organization, and specimen integrity across different experimental contexts.
Confocal Reflectance provides structural information from reflected or backscattered light without requiring fluorescence, whereas fluorescence-based methods depend on labeling. Using reflectance imaging alongside fluorescence can therefore add label-free information about cellular and tissue morphology. This is particularly relevant when researchers want to examine organization or specimen integrity without making fluorescence the sole source of contrast.