Fluorescent Voltage Sensor
Fluorescent Voltage Sensor - Related Videos
09:57
Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
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Cited by 5 •
2016
A method for imaging changes in membrane potential using genetically encoded voltage indicators is described.
09:24
Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids
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Cited by 13 •
2017
This article describes an enhancement of conventional Voltage-Clamp Fluorometry (VCF) where Fluorescent Unnatural Amino Acids (fUAA) are used instead of maleimide dyes, to probe structural rearrangements in ion channels. The procedure includes Xenopus oocyte DNA injection, RNA/fUAA coinjection, and simultaneous current and fluorescence measurements.
05:52
Measuring the Induced Membrane Voltage with Di-8-ANEPPS
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Cited by 48 •
2009
External electric field induces a voltage on the membrane of a cell, termed the induced membrane voltage (ΔΦ). By using the potentiometric dye di-8-ANEPPS, it is possible to measure the ΔΦ noninvasively. This video shows the protocol for measuring ΔΦ using di-8-ANEPPS.
10:23
Glutamine Flux Imaging Using Genetically Encoded Sensors
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Cited by 7 •
2014
This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine sensors.
05:15
An Aptamer-based Sensor for Unchelated Gadolinium(III)
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2017
The use of polydeoxynucleotide (44-mer aptamer) molecules for sensing unchelated gadolinium(III) ion in an aqueous solution is described. The presence of the ion is detected via an increase in the fluorescence emission of the sensor.
FAQs
A voltage change across the membrane can alter a sensor’s conformation, charge distribution, or retinal state. Those molecular changes modify the probe’s fluorescent output, creating an optical readout of electrical activity. Which molecular feature carries the response depends on the sensor’s design, but the common result is conversion of membrane physiology into measurable light.
Depolarization and hyperpolarization alter the electrical potential across the neuronal membrane in different directions, and each can change the sensor’s molecular state. The resulting fluorescence change reports that the membrane potential has shifted. Because the optical signal arises from voltage-dependent molecular changes, fluorescence imaging can track electrical events without relying solely on direct electrical recordings.
Their fluorescence responds to changes in membrane potential, not only to the threshold-crossing event of an action potential. Consequently, the same optical approach can reveal rapid neuronal firing as well as smaller voltage fluctuations that remain below threshold. This range helps connect individual electrical events with the broader membrane dynamics shaping neuronal communication.
These probes can report action potentials and subthreshold voltage dynamics in living neurons and neural circuits. That combination provides information about both discrete electrical events and ongoing changes in membrane state. Researchers can therefore examine neuronal communication at the level of individual activity patterns while also considering how those patterns operate within connected circuit contexts.
High spatial resolution allows optical measurements to distinguish activity across locations within living cells and neural circuits. This spatial information adds context that is important when studying circuit function, because electrical signaling can be related to particular cellular or circuit regions. The result is a view that links membrane physiology with the organization of neural activity.
Disease-related changes in electrical signaling can be examined through the fluorescence changes associated with altered membrane voltage. By applying optical measurements to living neurons or neural circuits, researchers can investigate how abnormal electrical activity relates to neuronal communication and circuit function. This makes the sensors useful for connecting changes in membrane physiology with broader disease-related neural effects.