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Biochemistry

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Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids
 

Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids

Article DOI: 10.3791/55598
May 27th, 2017

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Summary May 27th, 2017

This article describes an enhancement of conventional Voltage-Clamp Fluorometry (VCF) where Fluorescent Unnatural Amino Acids (fUAA) are used instead of maleimide dyes, to probe structural rearrangements in ion channels. The procedure includes Xenopus oocyte DNA injection, RNA/fUAA coinjection, and simultaneous current and fluorescence measurements.

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