Human Hippocampal Tissue
Human Hippocampal Tissue - Related Videos
03:35
Dissociating and Culturing Neurons from Hippocampal Tissue Samples
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2025
This video demonstrates the dissociation and culture of hippocampal neurons from hippocampal tissue samples using enzymatic and mechanical dissociations. The treatment with Ara-C inhibits the growth of non-neuronal cells, facilitating the selective growth of hippocampal neurons.
03:59
Formation and Expansion of Neurospheres from a Hippocampal Tissue
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2025
This video demonstrates the process of neurosphere formation and their expansion from hippocampal tissue. Neural stem and progenitor cells from the postnatal mouse dentate gyrus tissue proliferate and form primary neurospheres, which are then reseeded to form secondary neurospheres.
03:52
An Assay to Detect Autoantibodies in Human Serum using a Hippocampal Neuronal Culture
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2025
This video demonstrates a technique for identifying antibodies against specific antigens in a sample using a rat hippocampal neuronal culture. By exposing cultured neurons to patient samples containing autoantibodies targeting N-Methyl-D-aspartate receptors (NMDARs), the presence of autoantibodies bound to the neuronal surface is revealed through immunostaining.
03:42
Obtaining a Single-Cell Suspension from Mouse Hippocampal Tissue
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2025
This video demonstrates a protocol to obtain a highly viable single-cell suspension from the mouse brain hippocampus using a combination of automated mechanical dissociation and a mixture of enzymes to aid in enzymatic digestion.
03:25
Recording Neural Activity of Unfolded Hippocampal Tissue Using Penetrating Microelectrode Array
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2025
This video demonstrates the procedure to set up a recording chamber with a penetrating microelectrode array (PMEA) to capture neural signals from an unfolded hippocampus. This method allows us to study the speed and direction of propagation of neural activity within the unfolded hippocampus in vitro.
FAQs
These interconnected regions form a pathway through which information reaches and moves within the hippocampus. Inputs from the entorhinal cortex connect with the dentate gyrus, CA3, and CA1, creating an organized circuit rather than an isolated collection of cells. Examining this arrangement in human hippocampal tissue helps relate anatomical connectivity to learning, memory formation, and spatial navigation.
Synaptic plasticity refers to changes in the strength of communication between neurons. In hippocampal circuits, these changes provide a cellular basis for examining how experiences may be represented during learning and memory processes. Electrophysiological, molecular, and imaging approaches can investigate plasticity-related changes and connect them with the organization and function of hippocampal pathways.
Supporting cells are part of the cellular organization that researchers examine alongside hippocampal neurons. Their presence means that circuit function cannot be understood solely from neuronal connections or synaptic activity. Studying both cell populations in human hippocampal tissue provides a broader view of how cellular architecture relates to communication, learning, memory, and changes associated with neurological conditions.
Anatomical methods reveal cellular and circuit organization, while electrophysiological approaches examine neuronal communication and changes in synaptic strength. Molecular methods provide information about cellular features, and imaging methods help visualize structure or activity-related characteristics. Using these approaches together allows researchers to connect the dentate gyrus–CA3–CA1 pathway with functional and cellular findings.
Human tissue provides a direct reference for evaluating whether observations from experimental models correspond to human brain biology. Researchers can compare anatomical organization, neuronal communication, supporting-cell context, and synaptic plasticity across systems. This comparison helps identify which model findings may translate to human learning, memory, neurological disease, or age-related changes.
Investigations can relate hippocampal structure and function to epilepsy, Alzheimer’s disease, aging, and other neurological conditions. Anatomical, electrophysiological, molecular, and imaging findings may show how circuit organization or cellular communication differs in these contexts. Such results support a more detailed connection between disease-related changes and the hippocampal processes involved in learning and memory.