Mouse Astrocytes
Mouse Astrocytes - Related Videos
06:41
Astrocyte Isolation: A Method to Obtain Pure Preparation of Mouse Cortical Astrocytes
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2023
In this video, we describe the method to obtain a pure preparation of astrocytes from newly born mice.
11:25
Isolation and Culture of Mouse Cortical Astrocytes
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Cited by 460 •
2013
Astrocytes have been recognized to be versatile cells participating in fundamental biological processes that are essential for normal brain development and function, and central nervous system repair. Here we present a rapid procedure to obtain pure mouse astrocyte cultures to study the biology of this major class of central nervous system cells.
08:32
Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia
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Cited by 9 •
2016
Astrocytes are one of the most important key players in the central nervous system (CNS). Here, we are reporting a practical method of sexed hippocampal astrocyte culture protocol in order to study the mechanisms underlying the astrocyte function in male and female neonate pups after in-vitro ischemia.
02:12
Direct Neuronal Reprogramming of Mouse Astrocytes into Neurons
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2025
This video showcases the direct reprogramming of astrocytes expressing transforming factors into neurons. Transformation factors trigger a cascade of molecular events facilitating astrocyte-to-neuron conversion. The process is further supported by supplying specific agents and supplements through a neuronal differentiation medium.
04:46
Immunostaining of Fluorescently Labeled Astrocytes in a Mouse Brain Tissue Section
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2025
This video demonstrates the procedure for immunostaining a fixed mouse brain tissue section with fluorescently labeled astrocytes using primary and secondary antibodies and capture the images of the stained astrocytes through confocal microscopy.
FAQs
Their processes help control extracellular ion concentrations and neurotransmitter levels, limiting changes that could disrupt neuronal signaling. Astrocytes also contribute to energy availability, linking local metabolic support with neural activity. These functions allow researchers to examine how changes in homeostasis influence circuit behavior and synaptic plasticity in the central nervous system.
Astrocyte processes occupy positions that connect neural and vascular environments. Near synapses, they participate in local signaling conditions, while near blood vessels they support communication between neural and vascular cells. Studying these spatial relationships helps clarify how mouse astrocytes coordinate neuronal activity, tissue maintenance, and responses to changing physiological demands.
Changes in the brain or spinal cord can alter astrocyte activity and tissue responses. This makes these cells useful for investigating neuroinflammation and injury-related processes, as well as disorders such as epilepsy, neurodegeneration, and brain tumors. The resulting observations connect astrocyte behavior with both protective homeostatic functions and disease-associated changes.
Researchers examine mouse astrocytes in primary cultures, brain slices, transgenic models, and imaging experiments. Primary cultures provide an accessible preparation for studying astrocyte behavior, whereas brain slices preserve aspects of tissue organization. Transgenic models and imaging approaches extend these observations to astrocyte activity within neural circuits and intact experimental systems.
Imaging approaches can show where astrocyte processes are positioned relative to synapses and blood vessels and can help track activity associated with neural or vascular signaling. When combined with mouse models, imaging supports investigation of circuit activity, synaptic plasticity, and responses to injury or disease within more organized nervous-system contexts.
These models are valuable when a study needs to connect glial activity with neuronal function, tissue homeostasis, or disease-related change. Researchers can use cultures, slices, transgenic animals, or imaging according to the question being tested. Together, these approaches support studies of neural circuits, neuroinflammation, injury, epilepsy, neurodegeneration, and brain tumors.