Prominin-1 Marker
Prominin-1 Marker - Related Videos
07:04
Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum
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2020
Demonstrated here is an efficient and cost-effective method to purify, culture, and differentiate white matter stem cells from postnatal mouse cerebellum.
09:38
Multiplexed Immunofluorescence Assay for Spatial Assessment of Senescence Markers in vivo
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2026
Multiplexed immunofluorescence enables sequential labeling of multiple antibody markers within a single tissue section to support spatial analysis of tissue architecture and cell populations. Here, a workflow for multiplex detection of senescence-associated, structural, and immune markers is presented, including manual and automated slide preparation, iterative imaging, and single-cell analysis using digital pathology software.
12:03
The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
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Cited by 4 •
2011
The ability to produce transgenes for Caenorhabditis elegans using genomic DNA carried by fosmids is particularly attractive as all of the native regulatory elements are retained. Described is a simple and robust procedure for the production of transgenes via recombineering with the galK selectable marker.
08:45
Duplex Digital PCR for Simultaneous Quantification of Dual Genetic Markers
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2025
In this video, we demonstrate the duplex digital PCR (ddPCR) technique — a modification of the traditional PCR technique that is useful in detecting two different genetic markers simultaneously. A single PCR reaction is partitioned into nanoliter-sized emulsified droplets that are independently amplified, and the detection of differently colored fluorescence amplification signals from the fraction of droplets is used to compute the initial concentration of the target sequences.
11:22
Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1
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Cited by 3 •
2017
In this two-part study, a biological actuator was developed using highly flexible polydimethylsiloxane (PDMS) cantilevers and living muscle cells (cardiomyocytes), and characterized. The biological actuator was incorporated with a base made of modified PDMS materials to build a self-stabilizing, swimming biorobot.
FAQs
Prominin-1 expression provides a surface feature that researchers can measure across nervous-system cells, allowing populations with different levels of the marker to be identified or enriched. Its concentration in plasma-membrane protrusions and cholesterol-rich membrane domains contributes to this detectable distribution. The resulting groups support comparisons of neural stem and progenitor populations during development.
Prominin-1 does not distribute uniformly across the cell surface. Its concentration in plasma-membrane protrusions and cholesterol-rich membrane domains creates localized regions where antibody-based detection can recognize the protein. This membrane organization helps explain why surface expression can serve as a practical sorting feature, while also emphasizing that measurements reflect cellular organization and state.
Prominin-1 expression varies with cell state and tissue context, so a positive signal does not by itself establish that a cell is a neural stem cell. Researchers therefore interpret it alongside additional markers and functional assays. This combined approach distinguishes surface-marker enrichment from stronger evidence about developmental potential or stem-cell behavior.
A typical marker-based workflow detects Prominin-1 at the cell surface with an antibody and separates cells according to surface expression. The resulting Prominin-1-positive population can then be examined as an enriched group rather than assumed to be uniform. Additional markers and functional assays are used afterward to evaluate the population's neural identity and properties.
Interpretation should account for both the tissue being studied and the state of the cells, because Prominin-1 expression can change across contexts. A positive population may be useful for enrichment, but its biological meaning requires comparison with additional markers and functional measurements. This prevents marker status from being treated as a complete description of cell identity.
In nervous-system research, Prominin-1 supports the study of neural stem and progenitor-cell development by helping researchers enrich and examine selected populations. It is also used to investigate Prominin-1-positive subpopulations in brain tumors. These applications connect surface-marker analysis with developmental studies and tumor research, while retaining the need for complementary biological assays.