Labelling סלקטיבי של חלבונים על פני קרום התא באמצעות CyDye DIGE צבעים פלואוריד מינימלי

0:10

Introduction to Cell Surface Protein Labeling with Ettan DIGE System

1:12

Overview of the Experiment

2:19

Sample Preparation

3:13

Labeling with CyDye DIGE Fluors

4:03

Incubation and Quenching

4:45

Cell Lysis

6:31

First Dimension ISO-Electric Focusing

5:29

Rehydration of IPG Strips

7:27

Gel Casting

9:43

Second Dimension SDS-Page

7:55

Equilibration of IPG Strips

10:29

Gel Scanning

10:52

Results of Cell Surface Protein Labeling

11:21

Comparison with Fractionated Runs

12:30

2D DIGE Analysis

13:27

Conclusions