Takafumi Sakai Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering Saitama University Biography Publications Institution JoVE Articles Takafumi Sakai has not added a biography. If you are Takafumi Sakai and would like to personalize this page please email our Author Liaison for assistance. Publications Underlying Mechanism of the Cyclic Migrating Motor Complex in Suncus Murinus: a Change in Gastrointestinal PH is the Key Regulator Physiological Reports. Jan, 2017 | Pubmed ID: 28082431 The Effect of Glutamate on Ghrelin Release in Mice Cell Biology International. Mar, 2017 | Pubmed ID: 28067437 A Sexually Dimorphic Area of the Dorsal Hypothalamus in Mice and Common Marmosets Endocrinology. Dec, 2016 | Pubmed ID: 27726418 Molecular Cloning of Ghrelin and Characteristics of Ghrelin-Producing Cells in the Gastrointestinal Tract of the Common Marmoset (Callithrix Jacchus) Zoological Science. Oct, 2016 | Pubmed ID: 27715422 Identification of Marker Genes for Pars Tuberalis Morphogenesis in Chick Embryo: Expression of Cytokine-like 1 and Gap Junction Protein Alpha 5 in Pars Tuberalis Cell and Tissue Research. Dec, 2016 | Pubmed ID: 27590887 A Comparative Study of Sex Difference in Calbindin Neurons Among Mice, Musk Shrews, and Japanese Quails Neuroscience Letters. Sep, 2016 | Pubmed ID: 27531632 Longitudinal Investigation of Carriage Rates, Counts, and Genotypes of Toxigenic Clostridium Difficile in Early Infancy Applied and Environmental Microbiology. Oct, 2016 | Pubmed ID: 27451451 Early-Life Events, Including Mode of Delivery and Type of Feeding, Siblings and Gender, Shape the Developing Gut Microbiota PloS One. 2016 | Pubmed ID: 27362264 Molecular Cloning of Motilin and Mechanism of Motilin-induced Gastrointestinal Motility in Japanese Quail General and Comparative Endocrinology. Jul, 2016 | Pubmed ID: 27179882 The Proximal Gastric Corpus is the Most Responsive Site of Motilin-induced Contractions in the Stomach of the Asian House Shrew Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology. Jul, 2016 | Pubmed ID: 27062028 Involvement of Transient Receptor Potential Vanilloid Receptor 1, (TRPV1)-Expressing Vagal Nerve in the Inhibitory Effect of Gastric Acidification on Exogenous Motilin-Induced Gastric Phase III Contractions in Suncus Murinus Digestive Diseases and Sciences. Jun, 2016 | Pubmed ID: 26860510 Ghrelin Is an Essential Factor for Motilin-Induced Gastric Contraction in Suncus Murinus Endocrinology. Dec, 2015 | Pubmed ID: 26441238 A High-throughput Direct Fluorescence Resonance Energy Transfer-based Assay for Analyzing Apoptotic Proteases Using Flow Cytometry and Fluorescence Lifetime Measurements Analytical Biochemistry. Dec, 2015 | Pubmed ID: 26334608 Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus Murinus PloS One. 2015 | Pubmed ID: 26115342 Motilin Stimulates Pepsinogen Secretion in Suncus Murinus Biochemical and Biophysical Research Communications. Jul, 2015 | Pubmed ID: 25957475 Development of TaqMan-based Quantitative PCR for Sensitive and Selective Detection of Toxigenic Clostridium Difficile in Human Stools PloS One. 2014 | Pubmed ID: 25360662 Macrophage Colony-stimulating Factor Induces Prolactin Expression in Rat Pituitary Gland Zoological Science. Jun, 2014 | Pubmed ID: 24882100 Regulation of LH/FSH Expression by Secretoglobin 3A2 in the Mouse Pituitary Gland Cell and Tissue Research. Apr, 2014 | Pubmed ID: 24514953 G Protein-coupled Receptor 120 Signaling Regulates Ghrelin Secretion in Vivo and in Vitro American Journal of Physiology. Endocrinology and Metabolism. Jan, 2014 | Pubmed ID: 24222669 Mother-to-infant Transmission of Intestinal Bifidobacterial Strains Has an Impact on the Early Development of Vaginally Delivered Infant's Microbiota PloS One. 2013 | Pubmed ID: 24244304 Negative Regulation of Neuromedin U MRNA Expression in the Rat Pars Tuberalis by Melatonin PloS One. 2013 | Pubmed ID: 23843987 The Role of the Vagus Nerve in the Migrating Motor Complex and Ghrelin- and Motilin-induced Gastric Contraction in Suncus PloS One. 2013 | Pubmed ID: 23724093 Mechanism of Ghrelin-induced Gastric Contractions in Suncus Murinus (house Musk Shrew): Involvement of Intrinsic Primary Afferent Neurons PloS One. 2013 | Pubmed ID: 23565235 Ethnic Diversity of Gut Microbiota: Species Characterization of Bacteroides Fragilis Group and Genus Bifidobacterium in Healthy Belgian Adults, and Comparison with Data from Japanese Subjects Journal of Bioscience and Bioengineering. Aug, 2013 | Pubmed ID: 23522670 Detailed Morphogenetic Analysis of the Embryonic Chicken Pars Tuberalis As Glycoprotein Alpha Subunit Positive Region Journal of Molecular Histology. Aug, 2013 | Pubmed ID: 23269505 Ghrelin Increases Intracellular Ca²⁺ Concentration in the Various Hormone-producing Cell Types of the Rat Pituitary Gland Neuroscience Letters. Sep, 2012 | Pubmed ID: 22897875 In Vitro Selection of a Peptide Antagonist of Growth Hormone Secretagogue Receptor Using CDNA Display Proceedings of the National Academy of Sciences of the United States of America. Jul, 2012 | Pubmed ID: 22723348 Construction of Microbial Platform for an Energy-requiring Bioprocess: Practical 2'-deoxyribonucleoside Production Involving a C-C Coupling Reaction with High Energy Substrates Microbial Cell Factories. Jun, 2012 | Pubmed ID: 22709572 Molecular Identification of GHS-R and GPR38 in Suncus Murinus Peptides. Jul, 2012 | Pubmed ID: 22579813 Antagonistic Effect of Disulfide-rich Peptide Aptamers Selected by CDNA Display on Interleukin-6-dependent Cell Proliferation Biochemical and Biophysical Research Communications. Apr, 2012 | Pubmed ID: 22503683 Detailed Analysis of the δ-crystallin MRNA-expressing Region in Early Development of the Chick Pituitary Gland Journal of Molecular Histology. Jun, 2012 | Pubmed ID: 22461196 Coordination of Motilin and Ghrelin Regulates the Migrating Motor Complex of Gastrointestinal Motility in Suncus Murinus American Journal of Physiology. Gastrointestinal and Liver Physiology. May, 2012 | Pubmed ID: 22383491 Collision of Millimetre Droplets Induces DNA and Protein Transfection into Cells Scientific Reports. 2012 | Pubmed ID: 22375250 Immunocytochemical Localization of Kisspeptin Neurons in the Rat Forebrain with Special Reference to Sexual Dimorphism and Interaction with GnRH Neurons Endocrine Journal. 2012 | Pubmed ID: 22240892 Glutamine and Glutamic Acid Enhance Thyroid-stimulating Hormone β Subunit MRNA Expression in the Rat Pars Tuberalis The Journal of Endocrinology. Mar, 2012 | Pubmed ID: 22219301 Simple and Tunable Förster Resonance Energy Transfer-based Bioprobes for High-throughput Monitoring of Caspase-3 Activation in Living Cells by Using Flow Cytometry Biochimica Et Biophysica Acta. Feb, 2012 | Pubmed ID: 21791227 Transmission of Intestinal Bifidobacterium Longum Subsp. Longum Strains from Mother to Infant, Determined by Multilocus Sequencing Typing and Amplified Fragment Length Polymorphism Applied and Environmental Microbiology. Oct, 2011 | Pubmed ID: 21821739 Circadian Transcriptional Factor DBP Regulates Expression of Kiss1 in the Anteroventral Periventricular Nucleus Molecular and Cellular Endocrinology. Jun, 2011 | Pubmed ID: 21458520 Fermented Milk Containing Lactobacillus Casei Strain Shirota Reduces Incidence of Hard or Lumpy Stools in Healthy Population International Journal of Food Sciences and Nutrition. Jun, 2011 | Pubmed ID: 21322768 Fragments of Genomic DNA Released by Injured Cells Activate Innate Immunity and Suppress Endocrine Function in the Thyroid Endocrinology. Apr, 2011 | Pubmed ID: 21303947 Directed Evolution of a Three-finger Neurotoxin by Using CDNA Display Yields Antagonists As Well As Agonists of Interleukin-6 Receptor Signaling Molecular Brain. Jan, 2011 | Pubmed ID: 21214917 Ghrelin Cells in the Gastrointestinal Tract International Journal of Peptides. 2010 | Pubmed ID: 20798855 Physiological Characteristics of Gastric Contractions and Circadian Gastric Motility in the Free-moving Conscious House Musk Shrew (Suncus Murinus) American Journal of Physiology. Regulatory, Integrative and Comparative Physiology. Oct, 2010 | Pubmed ID: 20686171 M-RTLV Agar, a Novel Selective Medium to Distinguish Lactobacillus Casei and Lactobacillus Paracasei from Lactobacillus Rhamnosus International Journal of Food Microbiology. May, 2010 | Pubmed ID: 20385416 Screening and Characterization of a Phosphopentomutase Useful for Enzymatic Production of 2'-deoxyribonucleoside New Biotechnology. Oct, 2009 | Pubmed ID: 19818317 Identification of Ghrelin in the House Musk Shrew (Suncus Murinus): CDNA Cloning, Peptide Purification and Tissue Distribution Peptides. May, 2009 | Pubmed ID: 19428777 House Musk Shrew (Suncus Murinus, Order: Insectivora) As a New Model Animal for Motilin Study Peptides. Feb, 2009 | Pubmed ID: 18996160 Repeated-batch Production of Galactooligosaccharides from Lactose at High Concentration by Using Alginate-immobilized Cells of Sporobolomyces Singularis YIT 10047 The Journal of General and Applied Microbiology. Oct, 2008 | Pubmed ID: 19029770 Characteristic Features of Ghrelin Cells in the Gastrointestinal Tract and the Regulation of Stomach Ghrelin Expression and Production World Journal of Gastroenterology. Nov, 2008 | Pubmed ID: 19009644 Detailed Analysis of Formation of Chicken Pituitary Primordium in Early Embryonic Development Cell and Tissue Research. Sep, 2008 | Pubmed ID: 18584208 Primary Structure, Tissue Distribution, and Biological Activity of Chicken Motilin Receptor General and Comparative Endocrinology. May, 2008 | Pubmed ID: 18433751 Gastric Leptin, but Not Estrogen and Somatostatin, Contributes to the Elevation of Ghrelin MRNA Expression Level in Fasted Rats The Journal of Endocrinology. Mar, 2008 | Pubmed ID: 18310448 DNA Introduction into Living Cells by Water Droplet Impact with an Electrospray Process Angewandte Chemie (International Ed. in English). 2008 | Pubmed ID: 18203224 Hypophyseal Corticosteroids Stimulate Somatotrope Differentiation in the Embryonic Chicken Pituitary Gland Histochemistry and Cell Biology. Mar, 2008 | Pubmed ID: 18064482 Gastric Estrogen Directly Induces Ghrelin Expression and Production in the Rat Stomach The Journal of Endocrinology. Sep, 2006 | Pubmed ID: 17003276 Efficient Production of 2-deoxyribose 5-phosphate from Glucose and Acetaldehyde by Coupling of the Alcoholic Fermentation System of Baker's Yeast and Deoxyriboaldolase-expressing Escherichia Coli Bioscience, Biotechnology, and Biochemistry. Jun, 2006 | Pubmed ID: 16794316 One-pot Microbial Synthesis of 2'-deoxyribonucleoside from Glucose, Acetaldehyde, and a Nucleobase Biotechnology Letters. Jun, 2006 | Pubmed ID: 16786272 Identification of Immunoreactive Plasma and Stomach Ghrelin, and Expression of Stomach Ghrelin MRNA in the Bullfrog, Rana Catesbeiana General and Comparative Endocrinology. Sep, 2006 | Pubmed ID: 16630619 Biochemical Retrosynthesis of 2'-deoxyribonucleosides from Glucose, Acetaldehyde, and a Nucleobase Applied Microbiology and Biotechnology. Aug, 2006 | Pubmed ID: 16283293 Independent Differentiation of Mammotropes and Somatotropes in the Chicken Embryonic Pituitary Gland. Analysis by Cell Distribution and Attempt to Detect Somatomammotropes Histochemistry and Cell Biology. Apr, 2006 | Pubmed ID: 16215739 Identification, Cloning, and Characterization of a Sporobolomyces Singularis Beta-galactosidase-like Enzyme Involved in Galacto-oligosaccharide Production Journal of Bioscience and Bioengineering. Apr, 2005 | Pubmed ID: 16233798 Promoter Activity of Sea Lamprey Proopiocortin and Proopiomelanotropin Genes in AtT-20/D16v Cells General and Comparative Endocrinology. Nov, 2005 | Pubmed ID: 16039655 Exogenous Administration of Octanoic Acid Accelerates Octanoylated Ghrelin Production in the Proventriculus of Neonatal Chicks Biochemical and Biophysical Research Communications. Jul, 2005 | Pubmed ID: 15953586 Temporal and Spatial Expression of TGF-beta2 in Chicken Somites During Early Embryonic Development Journal of Experimental Zoology. Part A, Comparative Experimental Biology. May, 2005 | Pubmed ID: 15828015 Caspase-3 Sensitive Signaling in Vivo in Apoptotic HeLa Cells by Chemically Engineered Intramolecular Fluorescence Resonance Energy Transfer Mutants of Green Fluorescent Protein Biochemical and Biophysical Research Communications. May, 2005 | Pubmed ID: 15796904 Localization of Ghrelin-producing Cells in the Stomach of the Rainbow Trout (Oncorhynchus Mykiss) Zoological Science. Jul, 2004 | Pubmed ID: 15277719 Structural Determination and Histochemical Localization of Ghrelin in the Red-eared Slider Turtle, Trachemys Scripta Elegans General and Comparative Endocrinology. Aug, 2004 | Pubmed ID: 15242751 Development of Thyroid-stimulating Hormone Beta Subunit-producing Cells in the Chicken Embryonic Pituitary Gland Cells, Tissues, Organs. 2004 | Pubmed ID: 15237192 Development of Gonadotropes in the Chicken Embryonic Pituitary Gland Zoological Science. Apr, 2004 | Pubmed ID: 15118231 Estrogen Modulates Ghrelin Expression in the Female Rat Stomach Peptides. Feb, 2004 | Pubmed ID: 15063011 Construction of Deoxyriboaldolase-overexpressing Escherichia Coli and Its Application to 2-deoxyribose 5-phosphate Synthesis from Glucose and Acetaldehyde for 2'-deoxyribonucleoside Production Applied and Environmental Microbiology. Jul, 2003 | Pubmed ID: 12839746 Microbial Production of 2-deoxyribose 5-phosphate from Acetaldehyde and Triosephosphate for the Synthesis of 2'-deoxyribonucleosides Bioscience, Biotechnology, and Biochemistry. Apr, 2003 | Pubmed ID: 12784646 Growth Hormone Secretagogue Receptor Expression in the Cells of the Stomach-projected Afferent Nerve in the Rat Nodose Ganglion Neuroscience Letters. May, 2003 | Pubmed ID: 12757895 Existence of Ghrelin-immunopositive and -expressing Cells in the Proventriculus of the Hatching and Adult Chicken Regulatory Peptides. Mar, 2003 | Pubmed ID: 12609759 Appearance of Prolactin-releasing Peptide-producing Neurons in the Area Postrema of Adrenalectomized Rats Neuroscience Letters. Feb, 2003 | Pubmed ID: 12566169 Expression of PDS/Pds, the Pendred Syndrome Gene, in Endometrium The Journal of Clinical Endocrinology and Metabolism. Feb, 2002 | Pubmed ID: 11836344 Ghrelin-producing Cells Exist As Two Types of Cells, Closed- and Opened-type Cells, in the Rat Gastrointestinal Tract Peptides. Mar, 2002 | Pubmed ID: 11836003 Pituitary Folliculo-Stellate-Like Cells Stimulate Somatotroic Pituitary Tumor Growth in Nude Mice Endocrine Pathology. 1995 | Pubmed ID: 12114691 Safra Yolunun İnternalasyonunu İncelemek İçin Bir Tam Montajlı İmmünohistokimyasal Yöntem Kullanmak Ke Ren1, Yidan Dai1, Kai Yi2, Masanobu Kinoshita1, Masahiro Itoh2, Ichiro Sakata3, Takafumi Sakai3, Shuang-Qin Yi1 1Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 2Department of Anatomy, Tokyo Medical University, 3Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University JoVE 55483 Biologia
Safra Yolunun İnternalasyonunu İncelemek İçin Bir Tam Montajlı İmmünohistokimyasal Yöntem Kullanmak Ke Ren1, Yidan Dai1, Kai Yi2, Masanobu Kinoshita1, Masahiro Itoh2, Ichiro Sakata3, Takafumi Sakai3, Shuang-Qin Yi1 1Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 2Department of Anatomy, Tokyo Medical University, 3Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University JoVE 55483 Biologia