Real-Time RT-PCR for Gene Expression Tracking

Real-time RT-PCR tracks gene expression by measuring how much target DNA is made during PCR. It starts with mRNA, which is converted into complementary DNA, or cDNA, by an enzyme called reverse transcriptase. The cDNA is then amplified by PCR so the target sequence can be measured.

This method ca...

La reazione a catena della polimerasi a trascrizione inversa in tempo reale, o RT-PCR in tempo reale, può quantificare un RNA di interesse man mano che la reazione progredisce.

Per iniziare, la trascrittasi inversa copia l'RNA in cDNA complementare. La RNasi H digerisce l'RNA originale, lasciando dietro di sé piccoli primer attaccati al c...