Disease Tissue Analysis
Disease Tissue Analysis - Related Videos
08:11
Quantitative Analysis of Chromatin Proteomes in Disease
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Cited by 4 •
2012
Advances in mass spectrometry have allowed the high throughput analysis of protein expression and modification in a host of tissues. Combined with subcellular fractionation and disease models, quantitative mass spectrometry and bioinformatics can reveal new properties in biological systems. The method described herein analyzes chromatin-associated proteins in the setting of heart disease and is readily applicable to other in vivo models of human disease.
05:58
Tissue Triage and Freezing for Models of Skeletal Muscle Disease
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Cited by 98 •
2014
The analysis of skeletal muscle tissues to determine structural, functional, and biochemical properties is greatly facilitated by appropriate preparation. This protocol describes appropriate methods to prepare skeletal muscle tissue for a broad range of phenotyping studies.
07:42
Magnetic Levitation Coupled with Portable Imaging and Analysis for Disease Diagnostics
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Cited by 11 •
2017
We present a magnetic levitation technique coupled with automated imaging and analysis in both a smartphone-compatible device and a device with embedded imaging and processing. This is applied to measure the density distribution of cells with two demonstrated biomedical applications: sickle cell disease diagnosis and separating white and red blood cells.
01:23
Immunohistochemical Detection of Lewy Bodies in a Diseased Human Brain Tissue Section
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2025
Incubate with hydrogen peroxide solution to inhibit endogenous peroxidases and prevent non-specific staining.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
FAQs
Interpretation depends on connecting visible tissue architecture with molecular abnormalities. Histology and microscopy reveal altered cell morphology, while immunohistochemistry localizes changes in protein expression within tissue regions. Biochemical assays add measurements of enzyme activity and metabolites. Together, these layers can link a pathological pattern to a disease-associated molecular signature rather than relying on morphology alone.
Immunohistochemistry matters because it connects protein expression with specific tissue locations. The method can show whether a disease-associated protein is present, reduced, or distributed differently among cells or tissue regions. That spatial information complements biochemical measurements and helps relate molecular abnormalities to pathological changes, supporting disease distinction and biomarker assessment.
Measurements of enzyme activity and metabolites add biochemical evidence to structural and protein-level observations. An altered enzyme activity can indicate a functional molecular change, while an unusual metabolite pattern can contribute to a disease-associated signature. Used alongside tissue morphology and protein expression, these results help characterize composition and molecular change, strengthening disease assessment.
A tissue-based investigation can combine complementary observations rather than depending on one measurement. Researchers examine structure with histology and microscopy, assess protein distribution through immunohistochemistry, and measure enzyme activity or metabolites with biochemical assays. Comparing these findings allows the sample’s morphology, composition, and molecular changes to be considered together during disease evaluation.
Disease tissue analysis can support several decision points: distinguishing disease types, assessing progression, and evaluating responses to treatment. The relevant evidence may include altered cell morphology, protein expression, enzyme activity, metabolites, or other disease-associated biomarkers. Because these measurements connect tissue changes with molecular abnormalities, they provide an integrated basis for interpreting disease status.
In biochemistry, tissue-based measurements are valuable for investigating how molecular abnormalities relate to pathological changes. They support research into disease mechanisms and the development and validation of diagnostic biomarkers. The same evidence can inform targeted therapy studies and personalized approaches to patient care, linking experimental molecular findings with clinically relevant tissue patterns.