Airway Wall Model
Airway Wall Model - Related Videos
11:26
Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall
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Cited by 17 •
2015
Advancements in biomaterial technologies enable the development of three-dimensional multi-cell-type constructs. We have developed electrospinning protocols to produce three individual scaffolds to culture the main structural cells of the airway to provide a 3D in vitro model of the airway bronchiole wall.
09:10
Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease
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Cited by 7 •
2010
This video shows and compares two experimental models to study the development of obliterative airway disease (OAD) in mice, the heterotopic and orthotopic tracheal transplantation model.
11:21
Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
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Cited by 48 •
2010
This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.
12:30
Glycan Profiling of Plant Cell Wall Polymers using Microarrays
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Cited by 26 •
2012
A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.
12:24
A Novel Microsurgical Model for Heterotopic, En Bloc Chest Wall, Thymus, and Heart Transplantation in Mice
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Cited by 7 •
2016
To study combined solid organ and vascularized composite allotransplantation, we describe a novel heterotopic en bloc chest wall, thymus, and heart transplant model in mice using a cervical non-suture cuff technique.
FAQs
Layered organization lets different airway cell types contribute distinct functions within one engineered system. Epithelial cells support assessment of barrier behavior, while smooth muscle cells enable investigation of contraction. Extracellular matrix and biomaterial scaffolds help reproduce the surrounding structural context, allowing researchers to examine interactions among tissue components rather than studying each cell type in isolation.
Chemical and mechanical conditions act as controlled challenges that reveal how the reconstructed tissue responds. They can be selected to examine inflammation, contraction, barrier function, or remodeling, depending on the research question. Applying these conditions helps bioengineers connect an observed tissue response with a particular stimulus and compare how airway wall behavior changes across experimental settings.
These models provide a complementary way to investigate respiratory biology in a controlled engineered system. Researchers can focus on interactions among airway epithelial cells, smooth muscle cells, extracellular matrix, and scaffolds while applying defined conditions. Results can help clarify disease mechanisms and inform therapeutic development, while animal studies remain part of the broader research context rather than being replaced by one model.
Construction begins by combining the principal cellular and structural elements of the airway wall: airway epithelial cells, smooth muscle cells, extracellular matrix, and a biomaterial scaffold. Bioengineers organize these components to reproduce the wall’s layered arrangement, then expose the system to relevant chemical or mechanical conditions. This workflow creates a platform for evaluating defined tissue responses.
An Airway Wall Model can be used to assess several functional and pathological outcomes, including epithelial barrier function, inflammation, smooth muscle contraction, and tissue remodeling. Examining these responses under selected conditions helps researchers identify how airway-wall behavior changes during injury or disease. The resulting observations can support interpretation of respiratory mechanisms and evaluation of candidate interventions.
Airway wall models provide research platforms for studying asthma, chronic obstructive pulmonary disease, infection, and airway injury. Their engineered tissue context allows investigators to examine disease-associated responses and test potential drugs or therapeutic strategies. Because the systems can reproduce selected structural and functional features of the airway wall, they may also contribute to developing treatments that are more patient-relevant.