Spiral Microdevice
Spiral Microdevice - Related Videos
02:35
Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays
0 Views •
2025
This video demonstrates the process for isolating spiral ganglion explants from the inner ear and co-culturing them with the organ of Corti on a multielectrode array. Spiral ganglia are carefully extracted and sectioned into smaller tissue sections or explants. These explants are cultured on the multielectrode array's active area to promote neuronal process outgrowth.
03:51
Label-Free Neutrophil Separation from Tracheal Secretion Using Spiral Microfluidics Channel
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2025
This video demonstrates separating neutrophils from tracheal secretions using a microfluidic device with spiral channels. The device achieves efficient separation based on cell size by utilizing varying thicknesses and trapezoidal-shaped channels. The interplay between forces results in large neutrophils accumulating near the inner wall while erythrocytes and mucin aggregates remain near the outer wall, ensuring effective neutrophil separation.
07:51
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays
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Cited by 5 •
2016
We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.
11:34
Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice
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Cited by 29 •
2017
To study ovarian tumor progression in a physiologically relevant model, multicellular spheroids were cultured in a microdevice under simulated fluid flow. This dynamic 3D model emulates the intraperitoneal environment with the cellular and mechanical components where ovarian cancer metastasis occurs.
02:40
Analyzing Neural Activity in Primary Murine Spiral Ganglion Neurons Using Multielectrode Arrays
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2025
This video demonstrates the recording of spontaneous and stimulation-dependent neural activity from primary murine spiral ganglion neuron (SGN) cultures on a multielectrode array (MEA). It involves washing and preparing the MEA, recording spontaneous neural activity, and applying controlled electrical stimuli to identify stimulation-dependent neuronal activity.
FAQs
Separation arises from the interaction between two hydrodynamic effects. Inertial lift forces act on particles moving through the channel, while curvature generates Dean vortices, which are secondary circulating flows. Their combined action drives particles toward different equilibrium positions, allowing a spiral microdevice to distinguish and focus populations without labels.
Particle size and other particle properties influence how strongly inertial lift and Dean-vortex transport act on each particle. Because those effects do not position every particle identically, populations can focus at distinct locations within the curved channel. This size- or property-dependent positioning provides the physical basis for separating mixed biological samples.
Its passive, continuous-flow operation can provide a compact and low-cost alternative to centrifugation and other complex separation systems. It also supports label-free processing, so cells do not need an added labeling step for the separation mechanism described. These features are relevant when workflows prioritize rapid handling, reduced system complexity, and sample enrichment.
A sample is introduced into the curved microchannel and carried through it under flow. As the sample travels along the spiral, curvature produces Dean vortices while inertial lift acts on suspended particles or cells. The resulting positional differences focus distinct populations at separate locations, enabling continuous-flow separation or enrichment for downstream analysis.
In bioengineering, the platform can support blood analysis, circulating tumor cell isolation, and sample preparation. Its label-free enrichment capability is relevant when cell-containing samples must be processed without relying on labels as the separating principle. These uses connect microfluidic hydrodynamics with diagnostic workflows and research studies that require rapid handling of biological material.
The main outcome is the focusing, separation, or enrichment of cells and other particles according to their hydrodynamic behavior in the spiral channel. Because the process is continuous and label-free, it can prepare samples for later analysis while avoiding the need for a more complex separation setup. The compact format also suits rapid diagnostics and research workflows.