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Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays

Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays

DOI:
10.3791/54538-v

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07:51 min

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October 19, 2016

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, , , , ,
1Department of Otorhinolaryngology,Inselspital and University of Bern, 2Department of Clinical Research,Inselspital and University of Bern, 3Department of Physiology,University of Bern, 4Department of Clinical Neurosciences, Service ORL & HNS,University Hospital Geneva

Chapters

  • 00:05Title
  • 00:54Preparation of MEAs for Culture Experiments
  • 02:11Spiral Ganglion Dissection
  • 03:34Spiral Ganglion Explant Culture on MEAs
  • 04:37Electrophysiological Recordings to Investigate Spontaneous and Electrode Stimulation Dependent Activity
  • 05:52Results: Data Recordings on MEAs
  • 07:01Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.

Tags

Keywords: Spiral Ganglion NeuronExplant CultureMulti-electrode ArraysElectrophysiologyAuditory NeuronsCochlear ImplantECM CoatingBDNFSpiral LigamentSpiral GanglionOrgan Of CortiModiolus

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