Tumor Stromal
Tumor Stromal - Related Videos
05:48
Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion
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Cited by 7 •
2014
Tissue engineered fibroblast-derived native matrix is an emerging tool to generate a stromal substrate which supports epithelial cell proliferation and differentiation. Here a protocol applying this methodology to assess the impact of different stromal cell types on tumor cell biology is presented.
08:39
Three-dimensional Co-culture Model for Tumor-stromal Interaction
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Cited by 14 •
2015
Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts.
06:43
Robotic Duodenal Sleeve Resection for Gastrointestinal Stromal Tumor with Rare Exon 8 KIT Mutation Following Neoadjuvant Imatinib
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2026
This video case report demonstrates the unique management of a duodenal gastrointestinal stromal tumor with a rare, understudied, exon 8 KIT mutation. Neoadjuvant therapy resulted in a 30% reduction in tumor size, making the patient a candidate for a robotic-assisted duodenal sleeve resection instead of the more morbid pancreaticoduodenectomy.
13:01
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice
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Cited by 14 •
2011
In this report, we demonstrate a system to isolate and culture donor cells from the mouse mammary gland, and orthotopically transplant these cells in recipient mice to analyze stromal: epithelial interactions during mammary tumor development.
12:24
A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma
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Cited by 6 •
2021
Comprehensive in vitro models that faithfully recapitulate the relevant human disease are lacking. The current study presents three-dimensional (3D) tumor spheroid creation and culture, a reliable in vitro tool to study the tumor-stromal interaction in human hepatocellular carcinoma.
FAQs
Stromal fibroblasts influence malignant behavior through extracellular-matrix deposition and growth-factor signaling. By changing the surrounding matrix and local signaling environment, they can affect how tumor cells grow, invade nearby tissue, and respond to treatment. Bioengineered models that include fibroblasts therefore provide a way to examine cancer progression as an interaction between malignant cells and their supporting surroundings.
Endothelial cells contribute to angiogenesis, the formation of blood vessels within or around a tumor. This vascular activity is a key stromal process because it changes the tumor microenvironment and can influence tumor growth and treatment response. Including endothelial components in engineered systems helps researchers study how vessel-associated interactions contribute to disease behavior rather than examining cancer cells alone.
Immune cells participate in tumor stromal signaling and immune modulation, meaning they can change the local immune environment surrounding malignant cells. Their interactions with cancer cells and other stromal components may influence tumor progression and treatment response. Engineered tumor models can incorporate these interactions to investigate how the microenvironment affects cancer biology and to identify stromal pathways that may be therapeutically relevant.
These platforms recreate tumor stromal interactions in complementary ways. Three-dimensional cultures model cellular organization beyond a flat culture surface, organ-on-a-chip systems recreate interactions within an engineered platform, and engineered extracellular matrices focus on the surrounding structural environment. Together, they allow researchers to study tumor progression, invasion, signaling, and drug resistance under more biologically relevant conditions.
A typical approach is to recreate interactions among malignant cells, stromal fibroblasts, endothelial cells, immune cells, extracellular matrix, and signaling factors within a three-dimensional or engineered system. Researchers then examine outcomes such as tumor growth, invasion, and treatment response. This workflow connects defined microenvironmental components with measurable cancer behaviors and supports comparison of different stromal conditions.
These models are useful when treatment response depends on interactions between cancer cells and their surrounding microenvironment. Because stromal components can contribute to drug resistance, engineered systems may provide more predictive therapeutic testing than models focused only on malignant cells. They also help researchers identify stromal pathways that could be targeted alongside cancer cells to improve treatment strategies.