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A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma
This content is Free Access.
1Department of Biochemistry, Faculty of Pharmacy,Minia University, 2National Institute for Health Research Birmingham Liver Biomedical Research Unit and Centre for Liver and Gastrointestinal Research, Institute of Immunology and Immunotherapy,University of Birmingham, 3Newcastle Fibrosis Research Group, Biosciences Institute, Faculty of Medical Sciences,Newcastle University, 4Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences,Newcastle University, 5The Liver Unit, Department of Medicine, Freeman Hospital,Newcastle-upon-Tyne Hospitals NHS Foundation Trust
Chapters
- 00:05Introduction
- 01:20Cell Preparation
- 02:17Cell Collection and Counting
- 04:14Collection of Fibroblast Conditioned Media
- 05:17Heterotypic Tumor/Stromal Spheroids
- 06:29Homotypic Hep3B Spheroids in LX2 Conditioned Media
- 08:18Calculation of Spheroid Volume
- 09:17Results: In Vitro Creation and Culture of 3D Tumor Spheroid Model
- 11:28Conclusion
Comprehensive in vitro models that faithfully recapitulate the relevant human disease are lacking. The current study presents three-dimensional (3D) tumor spheroid creation and culture, a reliable in vitro tool to study the tumor-stromal interaction in human hepatocellular carcinoma.
