Scintillation Counter Measurement
Scintillation Counter Measurement - Related Videos
10:52
Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
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Cited by 21 •
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Phosphoinositides are signaling lipids whose relative abundance rapidly changes in response to various stimuli. This article describes a method to measure the abundance of phosphoinositides by metabolically labeling cells with 3H-myo-inositol, followed by extraction and deacylation. Extracted glycero-inositides are then separated by high-performance liquid chromatography and quantified by flow scintillation.
09:33
Determining Cell Number During Cell Culture using the Scepter Cell Counter
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The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.
05:01
Measuring Dopamine Uptake in Mouse Brain Synaptosomes Using Radiolabeled Dopamine
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This video demonstrates the assessment of dopamine uptake in synaptosomes harvested from mouse brains. It outlines steps for preparing synaptosomes for dopamine uptake via dopamine transporters, treating them with varying concentrations of radiolabeled dopamine, isolating the synaptosomes, and measuring radioactivity to quantify dopamine uptake.
13:16
Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter
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Cited by 18 •
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The Moxi Z miniature automated cell counter is a novel instrument that combines the Coulter Principle with patented thin-film sensor technology and a proprietary software algorithm to perform sizing and counting of a broad size range of particles as well as to determine the overall health of monodisperse mammalian cell cultures. This protocol describes the use of this instrument for counting and assessing the health of cell cultures.
04:25
Substrate Oxidation Assay in Cultured Cells: An In Vitro Assay to Quantify Substrate Oxidation in Cells by Measuring Radioactive Signals from Trapped CO2
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2025
This video demonstrates a quantification technique for CO2 released during substrate oxidation using 14C-radiolabeled substrates. The released 14CO2 is trapped in an alkaline solution and quantified by a scintillation counter. The oxidation of different substrates varies between tissues and reflects the pathophysiological condition of the tissue.
FAQs
Within the cocktail, emitted particles transfer energy to scintillator molecules, which then release photons. Photomultiplier tubes convert those light signals into an electrical detection signal that the instrument records. This sequence links radioactive decay to a measurable count and explains why the method can detect low levels of radiolabeled biological material.
Calibration establishes how instrument responses relate to radioactivity measurements, while quench correction improves quantitative accuracy during counting. Applying both is important when the goal is more than detecting a tracer, such as comparing radiolabeled material across samples. These adjustments help ensure that reported counts or disintegrations per minute support reliable interpretation.
The scintillator cocktail provides the energy-transfer medium surrounding the radiolabeled sample. Emitted particles deposit energy in this mixture, allowing the cocktail to produce photons that the detector can measure. This arrangement converts radiation from biological samples into a light-based signal, supporting sensitive quantification of labeled DNA, RNA, proteins, metabolites, or other tracers.
A researcher prepares the radiolabeled biological sample, mixes it with a scintillator cocktail, and places the resulting mixture into the counting workflow. The instrument detects photon-producing events and reports counts or disintegrations per minute. Calibration and quench correction are especially important when the measurements will be interpreted quantitatively or compared among samples.
The method can quantify radiolabeled DNA, RNA, proteins, metabolites, and cellular uptake. These measurements support investigations of metabolism, biosynthesis, transport, and ligand binding. The useful biological interpretation depends on which component carries the tracer, allowing radioactivity measurements to reflect labeled material associated with a particular cellular or molecular process.
Its high sensitivity makes the approach valuable when researchers need to analyze radiolabeled material in biological experiments. It can provide quantitative measurements for studies involving molecular components, metabolites, or cell-associated uptake. As a result, researchers can apply the method across diverse tracer investigations, including metabolism, biosynthesis, transport, and ligand-binding studies.