Ali Culture
Ali Culture - Related Videos
03:17
Viral Growth in Human Nasal Epithelial Cells Cultured at an Air-Liquid Interface
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2026
Source: Otter, C. J., et al. Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions. J. Vis. Exp. (2023)This video demonstrates the growth of coronavirus in human nasal epithelial cells cultured at an air-liquid interface, mimicking infection and viral replication in the human nasal cavity.
01:50
Establishing a Co-Culture Model to Study Host-Pathogen Interactions at the Air-Liquid Interface
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2026
Source: Montefusco-Pereira, C. V. et al. P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives. J. Vis. Exp.(2020)This video demonstrates a co-culture model of bronchial epithelial cells and macrophage-like cells at the air-liquid interface to study host-pathogen interactions. Pathogenic bacterial infection compromises epithelial membrane integrity, triggering macrophage-like cell activation and...
08:45
Method for Culture of Early Chick Embryos ex vivo (New Culture)
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Cited by 9 •
2008
This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Cited by 16 •
2015
This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.
08:47
Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System
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Cited by 10 •
2015
Here, we present an ex vivo flow model in which murine cardiac valves can be cultured allowing the study of the biology of the valve.
FAQs
Apical exposure to air creates a tissue-relevant condition that supports epithelial polarization, meaning the cells develop distinct apical and basal sides. This arrangement also promotes barrier formation, mucus production, and differentiation. As a result, the culture can reproduce several functional features of epithelial tissues more effectively than cells maintained with liquid covering both surfaces.
Polarization establishes directional organization within the epithelium, while barrier formation reflects the cells’ ability to create a tissue-like boundary. Together, these outcomes make the model more suitable for examining epithelial functions and responses. They are particularly relevant when researchers need to study processes involving the respiratory tract rather than undifferentiated cell growth alone.
The key difference is the condition at the apical surface. In ALI culture, that surface contacts air while nutrients are supplied from the basal side through liquid medium. This setup encourages mucus production, differentiation, polarization, and barrier development, whereas continuous liquid coverage does not provide the same air-exposed arrangement described for tissue-relevant epithelial modeling.
Cells, especially epithelial cells, are grown so that their basal surface remains supported by liquid medium while the apical medium is removed. The exposed apical surface then contacts air, and the basal side continues receiving nutrients. This arrangement is maintained to promote polarization, barrier formation, mucus production, and differentiation before the model is used for biological investigation.
Researchers choose this approach when they need an in vitro model that more closely reflects respiratory epithelial architecture and function. It can support investigations of epithelial development, host-pathogen interactions, inflammation, drug delivery, and inhaled toxicants. The air-exposed configuration is especially relevant for studying substances or biological events that affect the respiratory tract from its apical surface.
ALI cultures can reveal how epithelial tissues develop, differentiate, form barriers, produce mucus, and respond to pathogens or inflammatory conditions. They also provide a platform for evaluating drug delivery and inhaled toxicants in a tissue-relevant setting. By preserving important architecture and cell functions, the method can strengthen mechanistic studies and complement or reduce animal research.