Antibiotic-induced Donor
Antibiotic-induced Donor - Related Videos
02:30
F Plasmid Transfer and Dual Antibiotic Selection in E. coli
0 Views •
2025
Source: Erdogan, F.,et al., Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation. J. Vis. Exp. (2017)This video demonstrates the procedure for transferring an F plasmid from donor to recipient E. coli cells using a rescue plasmid and selecting transconjugants through dual antibiotic resistance. The process includes mixing bacterial cultures, incubation without shaking, disrupting mating pairs, and plating on selective media to confirm...
13:39
Antibiotic Susceptibility Testing: Epsilometer Tests to Determine MIC Values of Two Antibiotics and Evaluate Antibiotic Synergy
0 Views •
2023
Source: Anna Bläckberg1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund Sweden Knowledge of the interactions between antibiotics and bacteria is important in understanding how microbes evolve antibiotic resistance. In 1928, Alexander Fleming discovered penicillin, an antibiotic that exerts its antibacterial function by interfering with cell wall regeneration (1). Other antibiotics with diverse mechanisms of...
03:27
Assessing Antibiotic-Induced Filamentation in E. coli Using Plating Assay and Flow Cytometry
0 Views •
2025
Source: Cayron, J., and Lesterlin, C. Multi-scale Analysis of Bacterial Growth Under Stress Treatments. J. Vis. Exp. (2019)This video demonstrates the procedure for assessing E. coli filamentation and DNA replication under cell division-inhibiting antibiotic treatment using plating to measure viability and flow cytometry to analyze cell size and DNA content.
12:41
Stress-induced Antibiotic Susceptibility Testing on a Chip
0 Views •
Cited by 5 •
2014
We have developed a microfluidic platform for rapid antibiotic susceptibility testing. Fluid is passed at high speeds over bacteria immobilized on the bottom of a microfluidic channel. In the presence of stress and antibiotic, susceptible strains of bacteria die rapidly. However, resistant bacteria can survive these stressful conditions.
07:17
Single Port Donor Nephrectomy
0 Views •
Cited by 5 •
2011
Single port laparoscopic surgery is changing the standard of care in surgical care like nothing since the laparoscopic technique was introduced 20 years ago. We present out technique of single port donor nephrectomy using the Gelpoint device. We have successfully performed this surgery in 100 patients.
FAQs
Antibiotic stress can activate cellular responses in a susceptible bacterial system, increasing expression or mobilization of conjugative plasmids. This response may raise the donor cell’s capacity to produce the transfer machinery needed for DNA exchange. The key outcome is not simply survival under treatment, but altered genetic-transfer potential during drug pressure.
Conjugative plasmids are central because they carry genetic material that can be mobilized for transfer when the relevant cellular response is activated. Their increased expression or mobilization links antibiotic exposure to movement of DNA between bacteria. Studying this link helps distinguish drug pressure as a force that can influence transmission, rather than viewing resistance only as a pre-existing trait.
Mating pili provide the physical connection required for donor and recipient cells to exchange DNA through direct contact. When antibiotic exposure enhances the processes that support pilus formation, the opportunity for plasmid transfer may increase within a bacterial community. This makes pilus-associated contact an important observable step connecting cellular stress responses with horizontal gene transfer.
A study can compare donor behavior under antibiotic exposure with the corresponding susceptible system without that inducing pressure, then examine whether conjugative-plasmid mobilization and mating-pilus formation are enhanced. Researchers can also evaluate transfer to recipient cells through cell-to-cell contact. This workflow connects the treatment condition to the genetic-transfer outcome in the tested bacterial system.
These experiments can reveal whether antibiotic treatment changes plasmid transmission and the movement of resistance genes between bacterial cells. They also provide a way to examine how drug pressure shapes bacterial adaptation and interactions within microbial communities. The resulting evidence is relevant to resistance evolution because it links an environmental condition with altered horizontal gene transfer.
They help researchers examine how antimicrobial treatment may influence resistance-gene dissemination rather than considering resistance evolution separately from treatment conditions. By connecting antibiotic pressure with plasmid mobilization, cell-to-cell transfer, and bacterial adaptation, this system provides context for studying microbial communities. The findings can inform strategies intended to limit the spread of resistance.