Candida Albicans Mutants
Candida Albicans Mutants - Related Videos
03:50
Candida albicans Biofilm Formation in an In Vivo Mouse Model
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2025
This video demonstrates the development of an in vivo mouse model to study the biofilm formation of Candida albicans in subcutaneous tissue. The experiment involves implanting Candida-infected devices in the animal's back region for biofilm development.
08:05
Candida albicans Biofilm Chip (CaBChip) for High-throughput Antifungal Drug Screening
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Cited by 9 •
2012
We have developed a high-density microarray platform consisting of 3D nano-biofilms of C. albicans called CaBChip. The susceptibility profile of drugs tested on a CaBChip is comparable to the conventional 96-well plate model, suggesting that the fungal chip is ideally suited for true high-throughput screening of antifungal drugs.
08:31
Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging
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Cited by 26 •
2015
We present an experimental procedure of Candida albicans biofilm development in a mouse subcutaneous model. Fungal biofilms were quantified by determining the number of colony forming units and by a non-invasive bioluminescence imaging, where the amount of light that is produced corresponds with the number of viable cells.
11:09
Clarifying and Imaging Candida albicans Biofilms
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Cited by 7 •
2020
To view and quantify the internal features of Candida albicans biofilms, we prepare fixed intact specimens that are clarified by refractive index matching. Then, optical sectioning microscopy can be used to obtain three-dimensional image data though the full thickness of the biofilm.
07:19
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
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Cited by 105 •
2010
We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.
FAQs
Researchers compare a mutant with an otherwise similar reference strain under the same conditions. Differences in growth, morphology, stress response, virulence-related traits, or antifungal susceptibility can then be associated with the altered gene or its activity rather than with unrelated background variation. This comparison provides a framework for connecting genetic change with a specific biological outcome.
Morphology comparisons show whether a genetic change affects the forms that Candida albicans adopts during growth or interaction with its surroundings. Particular attention to the yeast-to-hyphae transition helps researchers examine how gene activity relates to a major virulence-related trait. Such results can clarify which genetic functions influence fungal behavior relevant to host interaction and pathogenesis.
A mutant that responds differently to environmental or drug-induced stress may carry a change affecting the fungus’s ability to tolerate that condition. Comparing these responses with a reference strain helps connect the altered gene or activity to stress handling or antifungal susceptibility. The findings can also indicate whether genetic variation may influence treatment responses.
Host interaction places fungal traits in a biologically relevant setting rather than examining growth alone. Mutant phenotypes linked to morphology, stress responses, or virulence-related characteristics can help explain how genetic changes influence relationships with the host. In biology, this context supports investigation of mechanisms contributing to opportunistic infection and fungal pathogenesis.
A typical investigation begins by creating or selecting strains with a genetic change, then comparing them with an otherwise similar reference strain. Researchers assess selected outcomes such as growth, morphology, stress responses, virulence-related traits, or antifungal susceptibility. Interpreting differences across these measurements helps associate the altered gene or activity with particular fungal processes.
Useful measurements include growth, morphology, stress responses, virulence-related traits, and susceptibility to antifungal treatment. Examining several phenotypes is valuable because a genetic change may affect more than one aspect of fungal biology. Together, the results can show whether the altered gene is associated primarily with development, stress handling, host-related behavior, or treatment response.
These strains help researchers investigate how genetic variation influences infection-related traits and responses to treatment. Findings may clarify mechanisms of fungal pathogenesis, identify genes or activities that could represent therapeutic targets, and show how altered susceptibility affects treatment outcomes. Their value comes from linking controlled genetic differences with measurable biological and clinically relevant phenotypes.