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JoVE Journal
Immunology and Infection

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Clarifying and Imaging Candida albicans Biofilms

Clarifying and Imaging Candida albicans Biofilms

Article DOI: 10.3791/60718-v 11:09 min March 6th, 2020
March 6th, 2020



To view and quantify the internal features of Candida albicans biofilms, we prepare fixed intact specimens that are clarified by refractive index matching. Then, optical sectioning microscopy can be used to obtain three-dimensional image data though the full thickness of the biofilm.



Candida Albicans Biofilms Clarification Methods Optical Sectioning Microscopy Confocal Scanning Fluorescence Microscopy Biofilm Growth 12-well Plate Cell Adhesion Filamentation Medium 48-hour Plate Culture Medium Fixative
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