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Chapters
- 00:04Introduction
- 00:48Growth of Biofilm Cultures
- 01:34Specimen Processing for Imaging
- 02:39Clarification Protocol: Biofilms on Impermeant Substrata
- 04:30Imaging Setup for Confocal Microscopy
- 05:41Fast Image Processing and Display for Large Confocal Image Stacks
- 08:21Results: Optimum Index Matching and Deep Imaging of Mutant and Wild Type C. albicans Biofilms
- 09:49Conclusion
To view and quantify the internal features of Candida albicans biofilms, we prepare fixed intact specimens that are clarified by refractive index matching. Then, optical sectioning microscopy can be used to obtain three-dimensional image data though the full thickness of the biofilm.
