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JoVE Journal
Immunology and Infection

This content is Open Access.

Clarifying and Imaging Candida albicans Biofilms
 

Clarifying and Imaging Candida albicans Biofilms

Article DOI: 10.3791/60718-v
March 6th, 2020

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Summary March 6th, 2020

To view and quantify the internal features of Candida albicans biofilms, we prepare fixed intact specimens that are clarified by refractive index matching. Then, optical sectioning microscopy can be used to obtain three-dimensional image data though the full thickness of the biofilm.

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