Cardiac Surface Imaging
Cardiac Surface Imaging - Related Videos
11:37
Cardiac Magnetic Resonance Imaging
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2023
Source: Frederick W. Damen and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana In this video, high field, small-bore magnetic resonance imaging (MRI) with physiological monitoring is demonstrated to acquire gated cine loops of the murine cardiovascular system. This procedure provides a basis for assessing left-ventricular function, visualizing vascular networks, and quantifying motion of organs due to respiration. Comparable small animal...
09:14
Cardiac Magnetic Resonance Imaging at 7 Tesla
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Cited by 10 •
2019
The sensitivity gain inherent to ultrahigh field magnetic resonance holds promise for high spatial resolution imaging of the heart. Here, we describe a protocol customized for functional cardiovascular magnetic resonance (CMR) at 7 Tesla using an advanced multi-channel radio-frequency coil, magnetic field shimming and a triggering concept.
03:02
Immunostaining of Bacteria-Filled Microlesions in a Mouse Cardiac Tissue Section for Imaging
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2026
Source: Brown, A. O., and Orihuela, C. J. Visualization of Streptococcus pneumoniae within Cardiac Microlesions and Subsequent Cardiac Remodeling. J. Vis. Exp. (2015)The video demonstrates the immunostaining of chemically fixed mouse cardiac tissue sections to visualize pathogenic bacteria localized within infection-induced microlesions. The process includes tissue washing, membrane permeabilization, blocking of nonspecific sites, antibody labeling, nuclear counterstaining, and confocal...
11:59
High-speed Particle Image Velocimetry Near Surfaces
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Cited by 19 •
2013
A procedure for studying transient flows near boundaries using high-resolution, high-speed particle image velocimetry (PIV) is described here. PIV is a non-intrusive measurement technique applicable to any optically accessible flow by optimizing several parameter constraints such as the image and recording properties, the laser sheet properties, and analysis algorithms.
12:15
Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
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Cited by 35 •
2009
Adult cardiac myocytes are primary cells that can be isolated from animal hearts and cultured for several days. Within this culture period adenoviral gene transfer can be used to express genetically encoded biosensors (GEBs) or fluorescent fusion proteins. Both approaches allow cellular investigations by means of confocal microscopy.
FAQs
Fluorescent indicators provide the signal link between cardiac activity and an image. When membrane voltage or intracellular calcium changes in epicardial tissue, the indicator’s emitted light changes correspondingly. A camera records these variations across the beating surface, allowing optical signals to represent dynamic electrical or calcium-related events rather than static anatomy.
Spatial analysis shows where activity occurs across the heart’s outer surface, whereas temporal analysis shows when regional changes occur during beating. Combining both dimensions helps researchers track conduction patterns, activation, and recovery. This makes it possible to identify differences between regions that might be missed by examining location or timing alone.
Membrane-voltage imaging focuses on changes associated with electrical activity, while intracellular-calcium imaging follows changes in calcium within cardiac cells. These signals provide complementary views of function at the heart surface. Comparing them can help researchers examine how electrical events and calcium-related activity are distributed across beating tissue.
A typical workflow places fluorescent indicators on the epicardium, records the beating surface with cameras, and analyzes the resulting image sequences. The recorded light changes are examined spatially and temporally to assess cardiac motion, electrical or calcium activity, conduction patterns, activation, and recovery. This sequence connects tissue preparation with interpretable functional measurements.
The method can reveal how activity propagates across the cardiac surface and whether activation or recovery differs between regions. Image analysis may also identify regional abnormalities in structure, motion, electrical behavior, or calcium activity. These outcomes give researchers a way to connect visible surface patterns with changes in cardiac physiology.
Cardiac Surface Imaging is useful for studying cardiac physiology and investigating mechanisms associated with arrhythmias and heart injury. It also supports evaluation of experimental therapies by showing how treatment-related changes appear across the heart surface. Because the approach captures regional and time-dependent behavior, it can compare functional patterns rather than relying only on overall observations.