Cd133 Positive Cells
Cd133 Positive Cells - Related Videos
05:40
Melanoma Stem Cell Isolation: A Method to Obtain CD133 Positive and Negative Melanoma Cells Using Magnetic-activated Cell Sorting
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2023
This video describes the technique of isolating CD133 negative and positive melanoma stem cells from a primary melanoma cell line using magnetic-activated cell sorting. The CD133 positive melanoma CSCs are further studied to understand the mechanisms of melanoma tumorigenesis.
12:06
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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Cited by 22 •
2011
Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.
12:16
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
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Cited by 22 •
2013
This article describes the preparation of freshly obtained melanoma tissue into primary cell cultures, and how to remove contaminations of erythrocytes and fibroblasts from the tumor cells. Finally, we describe how CD133+ putative melanoma stem cells are sorted from the CD133- bulk using Magnetic Activated Cell Sorting (MACS).
12:19
Analysis of Cell Cycle Position in Mammalian Cells
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Cited by 71 •
2012
Determining the cell cycle position of a population of cells, or understanding how signals affect proliferation, can be readily measured by flow cytometry using this protocol. We report a simple experimental approach to staining cells and quantifying their position in the cell cycle.
02:32
Position-effect Variegation
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2020
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
FAQs
CD133 expression serves as an indicator rather than definitive proof of stem-cell identity. Its presence can differ with tissue type, developmental state, and experimental conditions, so a CD133-positive population may contain biologically diverse cells. Researchers therefore interpret marker status alongside comparisons of heterogeneous populations and studies of their functional properties.
Researchers can label cells according to CD133 detection and then examine the positive and negative populations as separate groups. This comparison helps reveal whether the populations differ in functional properties or biological behavior. Because marker expression does not guarantee a single cell identity, analyzing both groups helps account for heterogeneity rather than treating positivity as a complete classification.
The proportion of CD133-positive cells may vary among tissues, across developmental states, and under different experimental conditions. These sources of variation can alter how a population appears in an experiment without necessarily indicating a change in definitive cell identity. Recording the tissue and study conditions is therefore important when interpreting or comparing results.
A typical workflow begins by detecting CD133 with labeled antibodies, followed by identifying the cells that carry the marker. Researchers may then enrich the positive population using antibody-based cell separation or use flow cytometry to examine and compare populations. The resulting positive and negative groups support downstream investigation of cellular heterogeneity and functional properties.
Flow cytometry provides a way to detect CD133-labeled cells and compare positive and negative populations, whereas antibody-based cell separation can enrich cells carrying the marker. These approaches serve complementary purposes: one supports population analysis, while the other helps obtain a more concentrated group for subsequent study. The selected method depends on the experimental goal.
Studies of CD133-positive cells contribute to research on tissue development, regeneration, and cancer biology. They also help investigators examine functional differences within heterogeneous cell populations. In these settings, CD133-based analysis can organize cells for comparison and enrichment, while variation in expression remains an important consideration when connecting marker status with biological properties.