Electrophysiology Rig
Electrophysiology Rig - Related Videos
05:41
Applying Microfluidics to Electrophysiology
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Cited by 1 •
2007
Microfluidics can be integrated with standard electrophysiology techniques to allow new experimental modalities. Specifically, the motivation for the ...
12:43
Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
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Cited by 12 •
2014
Innate defenses to virus infections are triggered by pattern recognition receptors (PRRs). The two cytoplasmic PRRs RIG-I and PKR bind to viral signature RNAs, change conformation, oligomerize, and activate antiviral signaling. Methods are described which allow to conveniently monitor the conformational switching and the oligomerization of these cytoplasmic PRRs.
10:46
Non-fluoroscopic Catheter Tracking for Fluoroscopy Reduction in Interventional Electrophysiology
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Cited by 4 •
2015
Radiation exposure is an underestimated risk in complex ablation procedures. Here, we describe a protocol to significantly decrease fluoroscopy time and dosage for both the patient and the lab staff by using a novel non-fluoroscopic catheter visualization system.
06:00
Forebrain Electrophysiological Recording in Larval Zebrafish
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Cited by 55 •
2013
A simple method to record extracellular field potentials in the larval zebrafish forebrain is described. The method provides a robust in vivo read-out of seizure-like activity. This technique can be used with genetically modified zebrafish larvae carrying epilepsy-related genes or seizures evoked by administration of convulsant drugs.
06:32
Electrophysiological Recording From Drosophila Labellar Taste Sensilla
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Cited by 28 •
2014
This protocol describes extracellular recording of the action potential responses fired by labellar taste neurons in Drosophila.
FAQs
Electrodes serve two complementary functions: they detect voltage changes generated by biological material, and they can inject controlled current into the preparation. This distinction allows researchers to observe spontaneous or experimentally evoked electrical behavior. Comparing responses before and after current injection can reveal how cells or tissues respond to controlled electrical conditions.
Bioelectric signals can be weak, so the rig uses amplifiers to increase their measurable strength before analysis. Data-acquisition equipment then converts the amplified activity into recordings that can be examined systematically. Together, these components help preserve electrical information that might otherwise be difficult to detect and support comparisons among cells, tissues, organs, or experimental conditions.
Electrical recordings can connect measured activity with several biological mechanisms, including neuronal signaling, muscle contraction, membrane properties, and ion-channel activity. Each application focuses on how electrical behavior reflects cellular function. This makes the approach useful for relating signal patterns or responses to underlying mechanisms rather than examining biological structure without its associated activity.
A basic workflow begins by positioning electrodes to detect activity or deliver controlled current, followed by amplification of the resulting bioelectric signals. Data-acquisition equipment converts those signals into recordings for analysis. The researcher can then compare electrical behavior across cells, tissues, or organs and evaluate how experimental conditions influence the measured response.
Researchers can use electrophysiology measurements when they need to determine how disease processes or drugs alter biological electrical activity. Recordings provide a way to compare electrical behavior under different experimental conditions and relate those changes to cellular mechanisms. This application is especially relevant when altered signaling, contraction, membrane behavior, or ion-channel activity is part of the investigation.
The same general setup supports investigations ranging from individual cellular properties to activity in tissues and organs. At the cellular level, measurements can address membranes or ion channels; at broader scales, they can examine neuronal signaling or muscle contraction. This range helps researchers connect local electrical events with coordinated biological function and communication.